{"doi":"10.1111/php.14014","title":"<scp>PpIX</scp>‐enabled fluorescence‐based detection and photodynamic priming of platinum‐resistant ovarian cancer cells under fluid shear stress","abstract":"Over 75% percent of ovarian cancer patients are diagnosed with advanced-stage disease characterized by unresectable intraperitoneal dissemination and the presence of ascites, or excessive fluid build-up within the abdomen. Conventional treatments include cytoreductive surgery followed by multi-line platinum and taxane chemotherapy regimens. Despite an initial response to treatment, over 75% of patients with advanced-stage ovarian cancer will relapse and succumb to platinum-resistant disease. Recent evidence suggests that fluid shear stress (FSS), which results from the movement of fluid such as ascites, induces epithelial-to-mesenchymal transition and confers resistance to carboplatin in ovarian cancer cells. This study demonstrates, for the first time, that FSS-induced platinum resistance correlates with increased cellular protoporphyrin IX (PpIX), the penultimate downstream product of heme biosynthesis, the production of which can be enhanced using the clinically approved pro-drug aminolevulinic acid (ALA). These data suggest that, with further investigation, PpIX could serve as a fluorescence-based biomarker of FSS-induced platinum resistance. Additionally, this study investigates the efficacy of PpIX-enabled photodynamic therapy (PDT) and the secretion of extracellular vesicles under static and FSS conditions in Caov-3 and NIH:OVCAR-3 cells, two representative cell lines for high-grade serous ovarian carcinoma (HGSOC), the most lethal form of the disease. FSS induces resistance to ALA-PpIX-mediated PDT, along with a significant increase in the number of EVs. Finally, the ability of PpIX-mediated photodynamic priming (PDP) to enhance carboplatin efficacy under FSS conditions is quantified. These preliminary findings in monolayer cultures necessitate additional studies to determine the feasibility of PpIX as a fluorescence-based indicator, and mediator of PDP, to target chemoresistance in the context of FSS.","journal":"Photochemistry and Photobiology","year":2024,"id":443131,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9454,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":618567,"name":"Brittany P. Rickard","orcid":"0000-0002-6112-1290","position":1,"is_corresponding":false},{"id":894282,"name":"Marta Overchuk","orcid":"0000-0002-8203-0427","position":2,"is_corresponding":false},{"id":729848,"name":"Prima Dewi Sinawang","orcid":"0000-0001-8460-3260","position":3,"is_corresponding":false},{"id":437740,"name":"Elizabeth E. Stanley","orcid":"0000-0002-6781-6747","position":4,"is_corresponding":false},{"id":752736,"name":"Matthew Mansi","orcid":null,"position":5,"is_corresponding":false},{"id":271802,"name":"Raymond G. Sierra","orcid":"0000-0002-6253-8282","position":6,"is_corresponding":false},{"id":847532,"name":"Brandon Hayes","orcid":null,"position":7,"is_corresponding":false},{"id":225652,"name":"Xianming Tan","orcid":"0000-0002-5478-2269","position":8,"is_corresponding":false},{"id":600836,"name":"Demir Akin","orcid":"0000-0002-2296-4674","position":9,"is_corresponding":false},{"id":942284,"name":"Bin Chen","orcid":"0000-0002-7736-4158","position":10,"is_corresponding":false},{"id":228637,"name":"Utkan Demirci","orcid":"0000-0003-2784-1590","position":11,"is_corresponding":false},{"id":282751,"name":"Imran Rizvi","orcid":"0000-0001-9673-4700","position":12,"is_corresponding":false},{"id":282747,"name":"Mustafa Kemal Ruhi","orcid":"0000-0003-3383-7782","position":0,"is_corresponding":true}],"reference_count":98,"raw_metadata":null,"created_at":"2026-07-19T02:01:24.471942Z","pmid":"39189505","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}