{"doi":"10.1111/php.13985","title":"Red light‐activated depletion of drug‐refractory glioblastoma stem cells and chemosensitization of an acquired‐resistant mesenchymal phenotype","abstract":"Glioblastoma stem cells (GSCs) are potent tumor initiators resistant to radiochemotherapy, and this subpopulation is hypothesized to re-populate the tumor milieu due to selection following conventional therapies. Here, we show that 5-aminolevulinic acid (ALA) treatment-a pro-fluorophore used for fluorescence-guided cancer surgery-leads to elevated levels of fluorophore conversion in patient-derived GSC cultures, and subsequent red light-activation induces apoptosis in both intrinsically temozolomide chemotherapy-sensitive and -resistant GSC phenotypes. Red light irradiation of ALA-treated cultures also exhibits the ability to target mesenchymal GSCs (Mes-GSCs) with induced temozolomide resistance. Furthermore, sub-lethal light doses restore Mes-GSC sensitivity to temozolomide, abrogating GSC-acquired chemoresistance. These results suggest that ALA is not only useful for fluorescence-guided glioblastoma tumor resection, but that it also facilitates a GSC drug-resistance agnostic, red light-activated modality to mop up the surgical margins and prime subsequent chemotherapy.","journal":"Photochemistry and Photobiology","year":2024,"id":462098,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1291555,"name":"Kohei Watanabe","orcid":"0000-0001-6519-5265","position":1,"is_corresponding":false},{"id":1291556,"name":"Megumi Ichikawa","orcid":"0000-0001-8916-0251","position":2,"is_corresponding":false},{"id":301747,"name":"Srivalleesha Mallidi","orcid":"0000-0003-1547-1764","position":3,"is_corresponding":false},{"id":1291557,"name":"Tatsuyuki Matsudaira","orcid":"0000-0003-2271-5317","position":4,"is_corresponding":false},{"id":1291975,"name":"Dmitriy Timerman","orcid":null,"position":5,"is_corresponding":false},{"id":494356,"name":"Joseph W. R. Swain","orcid":"0000-0002-5295-8523","position":6,"is_corresponding":false},{"id":743128,"name":"Zhiming Mai","orcid":"0000-0002-7819-9860","position":7,"is_corresponding":false},{"id":25157,"name":"Hiroaki Wakimoto","orcid":"0000-0001-8225-241X","position":8,"is_corresponding":false},{"id":233099,"name":"Tayyaba Hasan","orcid":"0000-0003-0871-6057","position":9,"is_corresponding":false},{"id":397926,"name":"Bryan Q. Spring","orcid":"0000-0001-7332-7707","position":0,"is_corresponding":true}],"reference_count":84,"raw_metadata":null,"created_at":"2026-07-19T02:04:20.631898Z","pmid":"38922889","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}