{"doi":"10.1111/php.12625","title":"Deficient Nucleotide Excision Repair in Squamous Cell Carcinoma Cells","abstract":"<jats:title>Abstract</jats:title><jats:p>Squamous cell carcinomas (<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>s) are associated with ultraviolet radiation and multiple genetic changes, but the mechanisms leading to genetic instability are unclear.<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>cell lines were compared to normal keratinocytes for sensitivity to ultraviolet radiation,<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content>repair kinetics and<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content>repair protein expression. Relative to normal keratinocytes, four<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>cell lines were all variably sensitive to ultraviolet radiation and, except for the<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>25 cell line, were deficient in global repair of cyclobutane pyrimidine dimers, although not 6‐4 photoproducts. Impaired<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content>repair of cyclobutane pyrimidine dimers was associated with reduced<jats:styled-content style=\"fixed-case\">mRNA</jats:styled-content>expression from<jats:italic><jats:styled-content style=\"fixed-case\">XPC</jats:styled-content></jats:italic>but not<jats:italic><jats:styled-content style=\"fixed-case\">DDB</jats:styled-content>2</jats:italic>genes which each encode key<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content>damage recognition proteins. However, levels of<jats:styled-content style=\"fixed-case\">XPC</jats:styled-content>or<jats:styled-content style=\"fixed-case\">DDB</jats:styled-content>2 proteins or both were variably reduced in repair‐deficient<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>cell lines. p53 levels did not correlate with<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content>repair activity or with<jats:styled-content style=\"fixed-case\">XPC</jats:styled-content>and<jats:styled-content style=\"fixed-case\">DDB</jats:styled-content>2 levels, but p63 levels were deficient in cell lines with reduced global repair. Repair‐proficient<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>25 cells depleted of p63 lost<jats:styled-content style=\"fixed-case\">XPC</jats:styled-content>expression, early global<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content>repair activity and<jats:styled-content style=\"fixed-case\">UV</jats:styled-content>resistance. These results demonstrate that some<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>cell lines are deficient in global nucleotide excision repair and support a role for p63 as a regulator of nucleotide excision repair in<jats:styled-content style=\"fixed-case\">SCC</jats:styled-content>s.</jats:p>","journal":"Photochemistry and Photobiology","year":2016,"id":36162,"datarank":0.19625308496161725,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.031461241661400796,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.031461241661400796,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":44.94283201407212,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":183703,"name":"Katherine Ona","orcid":null,"position":1,"is_corresponding":false},{"id":183704,"name":"Amy E. 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Dong","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27499003","pmcid":null,"openalex_id":"https://openalex.org/W2476504948","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"R01 CA105958","title":null},{"funder_name":"Biomedical Laboratory Research and Development, VA Office of Research and 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