{"doi":"10.1111/nph.71085","title":"The structure of intact and active Photosystem\n                    <scp>II</scp>\n                    from\n                    <i>Arabidopsis thaliana</i>\n                    at 2.44 Å resolution","abstract":"<jats:title>Summary</jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Photosystem II (PS II) is a large membrane‐bound protein complex that catalyses light‐driven water oxidation in plants and cyanobacteria. The structure of PS II is well studied in cyanobacteria; however, there are very few PS II structures from plants. The currently available plant PS II structures are comparatively low resolution and are frequently incomplete, that is, missing subunits or cofactors.</jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We optimized the procedure for isolating PS II from\n                          <jats:italic>Arabidopsis thaliana</jats:italic>\n                          and employed cryo‐electron microscopy to generate a high‐resolution structure of an intact and oxygen‐evolving PS II from\n                          <jats:italic>Arabidopsis thaliana</jats:italic>\n                          at 2.44 Å resolution, which to date represents the highest resolution structure of PS II from higher plants.\n                        </jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          At this resolution, many water molecules within the PS II structure can be detected, including waters around the water‐splitting manganese cluster, the nonheme iron, and within the water/proton channels connecting these active sites to the protein exterior, allowing for the first detailed description of the water networks in\n                          <jats:italic>Arabidopsis thaliana</jats:italic>\n                          and comparison with the highly resolved cyanobacterial PS II.\n                        </jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our findings further the understanding of design principles of protein–water–cofactor interactions in photosynthetic water splitting, quinone reduction/exchange, and about the role of lipids at the interface between PS II and the light‐harvesting proteins.</jats:p>\n                      </jats:list-item>\n                    </jats:list>\n                  </jats:p>","journal":"New Phytologist","year":2026,"id":651134,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1698061,"name":"André T. Graça","orcid":"0000-0002-5502-1744","position":1,"is_corresponding":false},{"id":1016487,"name":"A. Orkun Aydin","orcid":"0000-0002-6009-5297","position":2,"is_corresponding":false},{"id":785372,"name":"Michael Hall","orcid":"0000-0003-0864-9798","position":3,"is_corresponding":false},{"id":240865,"name":"Rana Hussein","orcid":"0000-0001-6292-2375","position":4,"is_corresponding":false},{"id":1698068,"name":"Wolfgang P. Schröder","orcid":"0000-0002-9492-5113","position":5,"is_corresponding":false},{"id":240890,"name":"Johannes Messinger","orcid":"0000-0003-2790-7721","position":6,"is_corresponding":false},{"id":1698059,"name":"Jack Forsman","orcid":"0000-0002-5111-8901","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The structure of intact and active Photosystem\n                    <scp>II</scp>\n                    from\n                    <i>Arabidopsis thaliana</i>\n                    at 2.44 Å resolution","abstract":"<jats:title>Summary</jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Photosystem II (PS II) is a large membrane‐bound protein complex that catalyses light‐driven water oxidation in plants and cyanobacteria. The structure of PS II is well studied in cyanobacteria; however, there are very few PS II structures from plants. The currently available plant PS II structures are comparatively low resolution and are frequently incomplete, that is, missing subunits or cofactors.</jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We optimized the procedure for isolating PS II from\n                          <jats:italic>Arabidopsis thaliana</jats:italic>\n                          and employed cryo‐electron microscopy to generate a high‐resolution structure of an intact and oxygen‐evolving PS II from\n                          <jats:italic>Arabidopsis thaliana</jats:italic>\n                          at 2.44 Å resolution, which to date represents the highest resolution structure of PS II from higher plants.\n                        </jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          At this resolution, many water molecules within the PS II structure can be detected, including waters around the water‐splitting manganese cluster, the nonheme iron, and within the water/proton channels connecting these active sites to the protein exterior, allowing for the first detailed description of the water networks in\n                          <jats:italic>Arabidopsis thaliana</jats:italic>\n                          and comparison with the highly resolved cyanobacterial PS II.\n                        </jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our findings further the understanding of design principles of protein–water–cofactor interactions in photosynthetic water splitting, quinone reduction/exchange, and about the role of lipids at the interface between PS II and the light‐harvesting proteins.</jats:p>\n                      </jats:list-item>\n                    </jats:list>\n                  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Forschungsgemeinschaft","grant_id":"SfB1078","title":null},{"funder_name":"Vetenskapsrådet","grant_id":"2020‐03809","title":null},{"funder_name":"Vetenskapsrådet","grant_id":"2024‐04804","title":null},{"funder_name":"Kempestiftelserna","grant_id":"JCK-2030","title":null},{"funder_name":"Kempestiftelserna","grant_id":"JCSMK24-599","title":null},{"funder_name":"Vetenskapsrådet","grant_id":"2024-04804","title":null},{"funder_name":"Vetenskapsrådet","grant_id":"2020-03809","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Sven och Lilly Lawskis Fond för Naturvetenskaplig Forskning","grant_id":"","title":null},{"funder_name":"Sven och Lilly Lawskis Fond för Naturvetenskaplig Forskning","grant_id":"","title":null}],"total_grants":13,"fwci":3.606,"citation_percentile":0.92058714,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1111/nph.71085","host_type":"journal"},{"url":"https://doi.org/10.1111/nph.71085","host_type":"publisher"},{"url":"https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.71085","host_type":"publisher"},{"url":"https://nph.onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.71085","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41958335","host_type":"repository"},{"url":"http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-252247","host_type":"repository"},{"url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-588245","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13150307/","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC13150307","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC13150307?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Photosynthetic Processes and Mechanisms","Metalloenzymes and iron-sulfur proteins","Mitochondrial Function and Pathology","0106 biological sciences","01 natural sciences","Photosystem II Protein Complex","Arabidopsis","Cryoelectron Microscopy","Models, Molecular","Water","Arabidopsis Proteins"],"mesh_terms":["Models, Molecular","Oxygen","Water","Arabidopsis","Cryoelectron Microscopy","Arabidopsis Proteins","Photosystem II Protein Complex"],"keywords":["Photosystem II","Arabidopsis thaliana","Arabidopsis","Resolution (logic)","Photosynthesis","Molecule","Photosystem I","Manganese","Manganese cluster","Water Channels","Photosystem Ii Structure","Cryo‐em","Protein–water–cofactor Interactions","Molekylärbiologi","Botany","Botanik","Biochemistry","protein-water-cofactor interactions","Structural Biology","Biokemi","Molecular Biology","Cryo-EM","Strukturbiologi"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T06:42:22.861636Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}