{"doi":"10.1111/mmi.14406","title":"Bacteriophage MS2 displays unreported capsid variability assembling <i>T</i> = 4 and mixed capsids","abstract":"<jats:title>Summary</jats:title><jats:p>Bacteriophage MS2 is a positive‐sense, single‐stranded RNA virus encapsulated in an asymmetric <jats:italic>T</jats:italic> = 3 pseudo‐icosahedral capsid. It infects <jats:italic>Escherichia coli</jats:italic> through the F‐pilus, in which it binds through a maturation protein incorporated into its capsid. Cryogenic electron microscopy has previously shown that its genome is highly ordered within virions, and that it regulates the assembly process of the capsid. In this study, we have assembled recombinant MS2 capsids with non‐genomic RNA containing the capsid incorporation sequence, and investigated the structures formed, revealing that <jats:italic>T</jats:italic> = 3, <jats:italic>T</jats:italic> = 4 and mixed capsids between these two triangulation numbers are generated, and resolving structures of <jats:italic>T</jats:italic> = 3 and <jats:italic>T</jats:italic> = 4 capsids to 4 Å and 6 Å respectively. We conclude that the basic MS2 capsid can form a mix of <jats:italic>T = </jats:italic>3 and <jats:italic>T = </jats:italic>4 structures, supporting a role for the ordered genome in favouring the formation of functional <jats:italic>T = </jats:italic>3 virions.</jats:p>","journal":"Molecular Microbiology","year":2020,"id":15568,"datarank":1.2142058311117347,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"self_citation_contribution":0.5416376868966337,"citation_network_contribution":0.6725681442151009,"self_endowment_contribution":0.5416376868966337,"citer_contribution":0.6725681442151009,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":31,"citers_with_citation_signal":27,"citers_with_endowment":27,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":118371,"name":"Michael A. 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Aylett","orcid":"0000-0003-0662-6664","position":5,"is_corresponding":false},{"id":118370,"name":"Natàlia de Martín Garrido","orcid":"0000-0001-7175-5919","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31618483","pmcid":"PMC7027807","openalex_id":"https://openalex.org/W2980952309","authors":[],"funders":[{"funder_name":"Royal Society","grant_id":"206212/Z/17/Z","title":null},{"funder_name":"Engineering and Physical Sciences Research Council","grant_id":"EP/R014000/1","title":"LCVD: Low-cost Cell-extract Viral Diagnostics"},{"funder_name":"Engineering and Physical Sciences Research Council","grant_id":"EP/S01778X/1","title":null},{"funder_name":"Medical Research Council","grant_id":"UKDRI-7001","title":null},{"funder_name":"Wellcome Trust","grant_id":"206212","title":"Understanding signal integration in eukaryotic cell growth"},{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":8,"fwci":2.775,"citation_percentile":0.90276643,"influential_citations":1,"citation_trend":[{"year":2020,"count":6},{"year":2021,"count":3},{"year":2022,"count":7},{"year":2023,"count":7},{"year":2024,"count":6},{"year":2025,"count":3},{"year":2026,"count":4}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.14406","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.14406","host_type":"HYBRID"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.14406","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.14406","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mmi.14406","host_type":"publisher"},{"url":"https://doi.org/10.1111/mmi.14406","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31618483","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7027807","host_type":"repository"},{"url":"http://hdl.handle.net/10044/1/74539","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7027807","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7027807?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1111/mmi.14406","host_type":""},{"url":"https://dx.doi.org/10.1111/mmi.14406","host_type":""},{"url":"https://doi.org/https://doi.org/10.1111/mmi.14406","host_type":""}],"fields_of_study":["Bacteriophages and microbial interactions","RNA and protein synthesis mechanisms","Viral gastroenteritis research and epidemiology","Medicine","Biology","Materials Science","0301 basic medicine","03 medical and health sciences","0303 health sciences","Capsid","Capsid Proteins","Cryoelectron Microscopy","Escherichia coli","Levivirus","Models, Molecular","RNA, Viral"],"mesh_terms":["Capsid","Escherichia coli","Models, Molecular","RNA, Viral","Levivirus","Cryoelectron Microscopy","Capsid Proteins"],"keywords":["Capsid","Bacteriophage","Biology","Bacteriophage MS2","Genome","RNA","Recombinant DNA","Icosahedral symmetry","Escherichia coli","Virology","Biophysics","Molecular biology","Genetics","Virus","Crystallography","Gene","Chemistry","Models, Molecular","Biochemistry & Molecular Biology","570","RNA CONTROLS","Microbiology","530","ARMORED RNA","DELIVERY","VIRUS-LIKE PARTICLES","07 Agricultural and Veterinary Sciences","REVEALS","CRYO-EM STRUCTURE","Triangulation number","3-DIMENSIONAL STRUCTURE","11 Medical and Health Sciences","Research Articles","Levivirus","Science & Technology","Cryoelectron Microscopy","Virion","DNA","06 Biological Sciences","Maturation protein","COAT PROTEIN","RNA, Viral","Capsid Proteins","Life Sciences & Biomedicine"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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