{"doi":"10.1111/mmi.13926","title":"Interference of transcription across H‐NS binding sites and repression by H‐NS","abstract":"<jats:title>Summary</jats:title><jats:p>Nucleoid‐associated protein H‐NS represses transcription by forming extended DNA–H‐NS complexes. Repression by H‐NS operates mostly at the level of transcription initiation. Less is known about how DNA–H‐NS complexes interfere with transcription elongation. <jats:italic>In vitro</jats:italic> H‐NS has been shown to enhance RNA polymerase pausing and to promote Rho‐dependent termination, while <jats:italic>in vivo</jats:italic> inhibition of Rho resulted in a decrease of the genome occupancy by H‐NS. Here we show that transcription directed across H‐NS binding regions relieves H‐NS (and H‐NS/StpA) mediated repression of promoters in these regions. Further, we observed a correlation of transcription across the H‐NS‐bound region and de‐repression. The data suggest that the transcribing RNA polymerase is able to remodel the H‐NS complex and/or dislodge H‐NS from the DNA and thus relieve repression. Such an interference of transcription and H‐NS mediated repression may imply that poorly transcribed AT‐rich loci are prone to be repressed by H‐NS, while efficiently transcribed loci escape repression.</jats:p>","journal":"Molecular Microbiology","year":2018,"id":600523,"datarank":0.5244761342199721,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":0.0,"self_endowment_contribution":0.5244761342199721,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1539614,"name":"Karin Schnetz","orcid":"0000-0003-4693-6202","position":1,"is_corresponding":false},{"id":981479,"name":"Aathmaja Anandhi Rangarajan","orcid":"0000-0003-3471-0257","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Interference of transcription across H‐NS binding sites and repression by H‐NS","abstract":"<jats:title>Summary</jats:title><jats:p>Nucleoid‐associated protein H‐NS represses transcription by forming extended DNA–H‐NS complexes. Repression by H‐NS operates mostly at the level of transcription initiation. Less is known about how DNA–H‐NS complexes interfere with transcription elongation. <jats:italic>In vitro</jats:italic> H‐NS has been shown to enhance RNA polymerase pausing and to promote Rho‐dependent termination, while <jats:italic>in vivo</jats:italic> inhibition of Rho resulted in a decrease of the genome occupancy by H‐NS. Here we show that transcription directed across H‐NS binding regions relieves H‐NS (and H‐NS/StpA) mediated repression of promoters in these regions. Further, we observed a correlation of transcription across the H‐NS‐bound region and de‐repression. The data suggest that the transcribing RNA polymerase is able to remodel the H‐NS complex and/or dislodge H‐NS from the DNA and thus relieve repression. Such an interference of transcription and H‐NS mediated repression may imply that poorly transcribed AT‐rich loci are prone to be repressed by H‐NS, while efficiently transcribed loci escape repression.</jats:p>","is_dataset_classified":null,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29424946","pmcid":null,"openalex_id":"https://openalex.org/W2789700864","authors":[],"funders":[{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/N014200/1","title":null},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/N005961/1","title":null},{"funder_name":"Universität zu Köln","grant_id":"","title":null}],"total_grants":3,"fwci":1.4435,"citation_percentile":0.81563102,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":7},{"year":2020,"count":3},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":3},{"year":2024,"count":5},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.13926","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.13926","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmmi.13926","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.13926","host_type":"publisher"},{"url":"https://doi.org/10.1111/mmi.13926","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29424946","host_type":"repository"}],"fields_of_study":["Genomics and Chromatin Dynamics","RNA Research and Splicing","Bacterial Genetics and Biotechnology"],"mesh_terms":["Bacterial Proteins","Binding Sites","DNA-Binding Proteins","DNA, Bacterial","Enzyme Repression","Escherichia coli","Promoter Regions, Genetic","DNA-Directed RNA Polymerases","Transcription, Genetic","Gene Expression Regulation, Bacterial","Escherichia coli Proteins"],"keywords":["Psychological repression","Transcription (linguistics)","Biology","Molecular biology","RNA polymerase II","RNA polymerase","DNA","Promoter","Nucleoid","RNA","Genetics","Gene","Gene expression","Escherichia coli"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Peace, Justice and strong institutions"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T13:23:47.587673Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}