{"doi":"10.1111/mmi.13901","title":"In <i>Streptomyces lividans</i>, acetyl‐CoA synthetase activity is controlled by <i>O‐</i>serine and <i>N<sup>ɛ</sup>‐</i>lysine acetylation","abstract":"<jats:title>Summary</jats:title><jats:p>Protein acetylation is a rapid mechanism for control of protein function. Acetyl‐CoA synthetase (AMP‐forming, Acs) is the paradigm for the control of metabolic enzymes by lysine acetylation. In many bacteria, type I or II protein acetyltransferases acetylate Acs, however, in actinomycetes type III protein acetyltransferases control the activity of Acs. We measured changes in the activity of the <jats:italic>Streptomyces lividans</jats:italic> Acs (<jats:italic>Sl</jats:italic>Acs) enzyme upon acetylation by PatB using <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> analyses. In addition to the acetylation of residue K610, residue S608 within the acetylation motif of <jats:italic>Sl</jats:italic>Acs was also acetylated (PKTRSGK<jats:sup>610</jats:sup>). S608 acetylation rendered <jats:italic>Sl</jats:italic>Acs inactive and non‐acetylatable by PatB. It is unclear whether acetylation of S608 is enzymatic, but it was clear that this modification occurred <jats:italic>in vivo</jats:italic> in <jats:italic>Streptomyces</jats:italic>. In <jats:italic>S. lividans</jats:italic>, an NAD<jats:sup>+</jats:sup>‐dependent sirtuin deacetylase from <jats:italic>Streptomyces</jats:italic>, SrtA (a homologue of the human SIRT4 protein) was needed to maintain <jats:italic>Sl</jats:italic>Acs function <jats:italic>in vivo</jats:italic>. We have characterized a sirtuin‐dependent reversible lysine acetylation system in <jats:italic>Streptomyces lividans</jats:italic> that targets and controls the Acs enzyme of this bacterium. These studies raise questions about acetyltransferase specificity, and describe the first Acs enzyme in any organism whose activity is modulated by <jats:italic>O‐</jats:italic>Ser and <jats:italic>N<jats:sup>ɛ</jats:sup>‐</jats:italic>Lys acetylation.</jats:p>","journal":"Molecular Microbiology","year":2018,"id":14584,"datarank":0.8204402783300664,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.35011614594069385,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.35011614594069385,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":16,"citers_with_citation_signal":14,"citers_with_endowment":14,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":114512,"name":"Jorge C. Escalante‐Semerena","orcid":"0000-0001-7428-2811","position":1,"is_corresponding":false},{"id":114510,"name":"Chelsey M. VanDrisse","orcid":"0000-0001-9139-0443","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29266439","pmcid":"PMC5796852","openalex_id":"https://openalex.org/W2775966897","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM062203","title":null},{"funder_name":"National Institute of General Medical Sciences","grant_id":"","title":null}],"total_grants":2,"fwci":1.1905,"citation_percentile":0.79129169,"influential_citations":0,"citation_trend":[{"year":2018,"count":2},{"year":2019,"count":4},{"year":2021,"count":4},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":6},{"year":2025,"count":3}],"oa_status":"green","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5796852","host_type":"repository"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.13901","host_type":"BRONZE"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5796852","host_type":"repository"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmmi.13901","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.13901","host_type":"publisher"},{"url":"https://doi.org/10.1111/mmi.13901","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29266439","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC5796852","host_type":"repository"}],"fields_of_study":["Microbial Natural Products and Biosynthesis","Enzyme Structure and Function","Microbial Metabolic Engineering and Bioproduction","Medicine","Biology","Chemistry","Acetate-CoA Ligase","Acetyl Coenzyme A","Acetylation","Acetyltransferases","Aminoacyltransferases","Bacterial Proteins","Cysteine Endopeptidases","DNA, Bacterial","Gene Deletion","Group III Histone Deacetylases","Lysine","NAD","Serine","Streptomyces lividans"],"mesh_terms":["Acetyl Coenzyme A","Acetate-CoA Ligase","Acetylation","Acetyltransferases","Bacterial Proteins","Cysteine Endopeptidases","DNA, Bacterial","Lysine","NAD","Serine","Gene Deletion","Aminoacyltransferases","Streptomyces lividans","Group III Histone Deacetylases"],"keywords":["Biology","Acetylation","Lysine","Serine","Biochemistry","Streptomyces","Enzyme","Genetics","Bacteria","Amino acid","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"brenda"},{"name":"pfam"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T12:29:55.943232Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}