{"doi":"10.1111/jvp.12694","title":"Expression of functional<scp>TRPV</scp>1 receptor in primary culture of canine keratinocytes","abstract":"<jats:title>Abstract</jats:title><jats:p>The interest for the endovanilloid system and for transient receptor potential vanilloid 1 (<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1) is continuously increasing, due to their involvement in inflammation, nociception and pruritus. Even if<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1 enrolment was highlighted in both physiological and pathological conditions, some aspects remain unclear, mostly in veterinary medicine. This study aimed to verify the expression and functionality of<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1 in canine keratinocytes to investigate in vitro the role of<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1 in these cells that are involved in different cutaneous pathologies. Keratinocytes primary cultures were isolated from bioptical samples and cultivated. Binding assay (using<jats:sup>3</jats:sup>[H]‐resiniferatoxin), displacement assay (in the presence of 1.2 <jats:styled-content style=\"fixed-case\">nM</jats:styled-content><jats:sup>3</jats:sup>[H]‐resiniferatoxin) and functional assays (in the presence of 1 μCi/<jats:sup>45</jats:sup>Ca<jats:sup>2+</jats:sup>) with vanilloid agonists and antagonists, specifically addressed to<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1 receptor, were performed. Binding assay demonstrated the presence of measurable concentrations of<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1 (<jats:italic>B</jats:italic><jats:sub>max</jats:sub> = 1,240 ± 120 fmol/mg protein;<jats:italic>K</jats:italic><jats:sub>d</jats:sub> = 0.01 ± 0.004 <jats:styled-content style=\"fixed-case\">nM</jats:styled-content>). Displacement assay highlighted the highest affinity for resiniferatoxin (<jats:styled-content style=\"fixed-case\">RTX</jats:styled-content>) and 5‐iodo‐resiniferatoxin (5‐I‐<jats:styled-content style=\"fixed-case\">RTX</jats:styled-content>), among agonists and antagonists, respectively. The same compounds results as the most potent in the functional assays. This study demonstrated the identification and the characterization of<jats:styled-content style=\"fixed-case\">TRPV</jats:styled-content>1 receptor in primary canine keratinocytes cultures. The results are promising for a clinical use, but further in vivo investigations are required.</jats:p>","journal":"Journal of Veterinary Pharmacology and Therapeutics","year":2018,"id":27427,"datarank":0.45572601166655874,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.09604172074680306,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.09604172074680306,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":6,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":156331,"name":"Cristina Vercelli","orcid":"0000-0001-7766-4647","position":1,"is_corresponding":false},{"id":156332,"name":"Barbara Cuniberti","orcid":null,"position":2,"is_corresponding":false},{"id":156333,"name":"Maria Federica della Valle","orcid":null,"position":3,"is_corresponding":false},{"id":156334,"name":"Marina Martano","orcid":null,"position":4,"is_corresponding":false},{"id":156335,"name":"Giovanni Re","orcid":null,"position":5,"is_corresponding":false},{"id":156330,"name":"Raffaella Barbero","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30043987","pmcid":null,"openalex_id":"https://openalex.org/W2884107038","authors":[],"funders":[{"funder_name":"Università degli Studi di Torino","grant_id":"","title":null},{"funder_name":"Regione Piemonte","grant_id":"","title":null},{"funder_name":"INNOVET ITALIA srl","grant_id":"","title":null},{"funder_name":"Regione Piemonte","grant_id":"","title":null},{"funder_name":"INNOVET ITALIA srl","grant_id":"","title":null},{"funder_name":"Università degli Studi di Torino","grant_id":"","title":null}],"total_grants":6,"fwci":0.5479,"citation_percentile":0.60269483,"influential_citations":0,"citation_trend":[{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2885","host_type":"repository"},{"url":"https://iris.unito.it/bitstream/2318/1685257/1/OPEN%20ACCESS%20Expression%20of%20functional%20TRPV1%20receptors%20in%20primary%20culture%20of%20canine%20keratinocytes.pdf","host_type":"GREEN"},{"url":"http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2885","host_type":"repository"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjvp.12694","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/jvp.12694","host_type":"publisher"},{"url":"https://doi.org/10.1111/jvp.12694","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30043987","host_type":"repository"}],"fields_of_study":["Ion Channels and Receptors","Dermatology and Skin Diseases","Pain Mechanisms and Treatments","Medicine","Chemistry","Biology","Anilides","Animals","Capsaicin","Cells, Cultured","Cinnamates","Diterpenes","Dogs","Gene Expression Regulation","Keratinocytes","TRPV Cation Channels"],"mesh_terms":["Anilides","Animals","Capsaicin","Cells, Cultured","Cinnamates","Diterpenes","Dogs","Gene Expression Regulation","Keratinocytes","TRPV Cation Channels"],"keywords":["Resiniferatoxin","TRPV","Chemistry","Pharmacology","Receptor","Agonist","Transient receptor potential channel","Biochemistry","TRPV1","Medicine","Characterization","Identification","Keratinocytes","Dog"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T18:01:06.621000Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}