{"doi":"10.1111/jvim.70259","title":"Evaluation of a Rapid, Low-Cost Broth Turbidity Test for Detecting Ampicillin-Resistant Lower Urinary Tract Infections in Dogs and Cats","abstract":"BACKGROUND: Effective treatment of urinary tract infections (UTIs) in dogs and cats relies on timely recognition of antimicrobial resistance, but traditional microbiological culture and susceptibility testing require 48-72 h and can be cost-prohibitive. OBJECTIVE: To evaluate a novel, low-cost broth turbidity test for detecting ampicillin-resistant UTIs in dogs and cats compared to the gold standard microbiological methods. ANIMALS: One hundred sixty urine samples from 145 dogs and 15 cats at the Mississippi State University College of Veterinary Medicine. METHODS: From September 2023 to February 2024, urine samples were tested using an ampicillin-supplemented Mueller-Hinton broth turbidity test and gold standard culture with Sensititre MIC plates. Sensitivity, specificity, Cohen's κ, and McNemar's test were calculated. RESULTS: The broth turbidity test showed 89.47% sensitivity (95% CI: 66.86%-98.72%) and 100% specificity (95% CI: 97.42%-100%) for the detection of ampicillin-resistant organisms in the urine, with substantial agreement (κ = 0.94, p < 0.001) and no classification difference (p = 0.500). Notably, all 36 samples with ampicillin-susceptible organisms identified by the gold standard cultures test were correctly identified as negative for ampicillin resistance by the broth turbidity test. CONCLUSIONS: The broth turbidity test's simplicity and affordability make it a promising tool for in-house use by veterinary practitioners to guide first-line therapy decisions, though its sensitivity could be limited in cases with low bacterial loads or confounding factors such as recent antibiotic administration.","journal":"Journal of Veterinary Internal Medicine","year":2025,"id":576643,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9615,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1432817,"name":"Larry D. Ballard","orcid":null,"position":1,"is_corresponding":false},{"id":1485874,"name":"Anna Collinsgru","orcid":null,"position":2,"is_corresponding":false},{"id":1485875,"name":"Josh Burnette","orcid":null,"position":3,"is_corresponding":false},{"id":1485876,"name":"Khadija Ferdous","orcid":null,"position":4,"is_corresponding":false},{"id":1485877,"name":"Joo You Park","orcid":null,"position":5,"is_corresponding":false},{"id":486884,"name":"Keun Seok Seo","orcid":"0000-0001-9280-9680","position":6,"is_corresponding":false},{"id":1432468,"name":"W. Cooper Brookshire","orcid":"0000-0002-8943-8388","position":0,"is_corresponding":true}],"reference_count":15,"raw_metadata":null,"created_at":"2026-07-19T02:58:00.620755Z","pmid":"41035374","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}