{"doi":"10.1111/jnc.15877","title":"Single‐cell <scp>RNA</scp>‐seq uncovers novel metabolic functions of Schwann cells beyond myelination","abstract":"Schwann cells (SCs) support peripheral nerves under homeostatic conditions, independent of myelination, and contribute to damage in prediabetic peripheral neuropathy (PN). Here, we used single-cell RNA sequencing to characterize the transcriptional profiles and intercellular communication of SCs in the nerve microenvironment using the high-fat diet-fed mouse, which mimics human prediabetes and neuropathy. We identified four major SC clusters, myelinating, nonmyelinating, immature, and repair in healthy and neuropathic nerves, in addition to a distinct cluster of nerve macrophages. Myelinating SCs acquired a unique transcriptional profile, beyond myelination, in response to metabolic stress. Mapping SC intercellular communication identified a shift in communication, centered on immune response and trophic support pathways, which primarily impacted nonmyelinating SCs. Validation analyses revealed that neuropathic SCs become pro-inflammatory and insulin resistant under prediabetic conditions. Overall, our study offers a unique resource for interrogating SC function, communication, and signaling in nerve pathophysiology to help inform SC-specific therapies.","journal":"Journal of Neurochemistry","year":2023,"id":332041,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9505,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":850009,"name":"Mohamed H. Noureldein","orcid":"0000-0002-7021-1511","position":1,"is_corresponding":false},{"id":304442,"name":"Bhumsoo Kim","orcid":"0000-0002-2645-1673","position":2,"is_corresponding":false},{"id":489886,"name":"Lucy M. Hinder","orcid":"0000-0002-5206-8010","position":3,"is_corresponding":false},{"id":483167,"name":"Faye E. Mendelson","orcid":"0000-0002-5601-7675","position":4,"is_corresponding":false},{"id":483168,"name":"John M. Hayes","orcid":"0000-0002-5677-2216","position":5,"is_corresponding":false},{"id":350442,"name":"Junguk Hur","orcid":"0000-0002-0736-2149","position":6,"is_corresponding":false},{"id":255269,"name":"Eva L. Feldman","orcid":"0000-0002-9162-2694","position":7,"is_corresponding":false},{"id":350440,"name":"Stéphanie Eid","orcid":"0000-0003-3775-7544","position":0,"is_corresponding":true}],"reference_count":103,"raw_metadata":null,"created_at":"2026-07-19T01:09:25.015646Z","pmid":"37328915","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}