{"doi":"10.1111/jnc.12460","title":"Genomic editing opens new avenues for zebrafish as a model for neurodegeneration","abstract":"<jats:title>Abstract</jats:title><jats:p>Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator‐like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat‐associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock‐out and, importantly, also makes knock‐in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off‐target effects and toxicity as frequently seen in conventional knock‐down approaches.</jats:p>","journal":"Journal of Neurochemistry","year":2013,"id":632377,"datarank":0.6141516843333151,"base_score":4.0943445622221,"endowment":4.0943445622221,"self_citation_contribution":0.6141516843333151,"citation_network_contribution":0.0,"self_endowment_contribution":0.6141516843333151,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":59,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":84331,"name":"Christian Haass","orcid":"0000-0002-4869-1627","position":1,"is_corresponding":false},{"id":80171,"name":"Bettina Schmid","orcid":"0000-0001-8043-7269","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Genomic editing opens new avenues for zebrafish as a model for neurodegeneration","abstract":"<jats:title>Abstract</jats:title><jats:p>Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator‐like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat‐associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock‐out and, importantly, also makes knock‐in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off‐target effects and toxicity as frequently seen in conventional knock‐down approaches.</jats:p>","is_dataset_classified":null,"base_score":4.0943445622221,"endowment":4.0943445622221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24117801","pmcid":null,"openalex_id":"https://openalex.org/W2054043430","authors":[],"funders":[{"funder_name":"European Research Council","grant_id":"FP7/2007-2013","title":null},{"funder_name":"European Research Council","grant_id":"321366-Amyloid","title":null},{"funder_name":"European Commission","grant_id":"321366","title":"Identification and modulation of pathogenic Amyloid beta-peptide species"}],"total_grants":3,"fwci":3.3031,"citation_percentile":0.92884882,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":9},{"year":2015,"count":6},{"year":2016,"count":7},{"year":2017,"count":5},{"year":2018,"count":10},{"year":2019,"count":3},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":4},{"year":2025,"count":2}],"oa_status":"bronze","license":"Wiley Online Library User Agreement","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jnc.12460","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jnc.12460","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjnc.12460","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/jnc.12460","host_type":"publisher"},{"url":"https://doi.org/10.1111/jnc.12460","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24117801","host_type":"repository"},{"url":"https://pub.dzne.de/search?p=id:%22DZNE-2020-03426%22","host_type":"repository"},{"url":"https://dx.doi.org/10.1111/jnc.12460","host_type":""},{"url":"https://pub.dzne.de/record/137104","host_type":""},{"url":"http://dx.doi.org/10.1111/jnc.12460","host_type":""}],"fields_of_study":["CRISPR and Genetic Engineering","Zebrafish Biomedical Research Applications","Neurogenetic and Muscular Disorders Research","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","Deoxyribonucleases","Disease Models, Animal","Genetic Engineering","Humans","Zebrafish","Zinc Fingers","Genome","Neurodegenerative Diseases","Animals, Genetically Modified","Gene Knockout Techniques","Gene Knock-In Techniques","Clustered Regularly Interspaced Short Palindromic Repeats"],"keywords":["Zebrafish","Genome editing","Transcription activator-like effector nuclease","Model organism","Zinc finger nuclease","Palindrome","Biology","Computational biology","Effector","CRISPR","Genome","Genetics","Organism","Neurodegeneration","Gene","Cell biology","Medicine","Genomics","Talen","Crispr/cas (Clustered Regularly Interspaced Short Palindromic Repeats)","Genetic Engineering: methods","Deoxyribonucleases","Neurodegenerative Diseases: genetics","Neurodegenerative Diseases","Zinc Fingers","Animals, Genetically Modified","Disease Models, Animal","Gene Knockout Techniques","Zebrafish: genetics","Animals","Humans","Clustered Regularly Interspaced Short Palindromic Repeats","Gene Knock-In Techniques","Genetic Engineering","Deoxyribonucleases: genetics","info:eu-repo/classification/ddc/610"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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