{"doi":"10.1111/jmi.12220","title":"A combined light sheet fluorescence and differential interference contrast microscope for live imaging of multicellular specimens","abstract":"<jats:title>Summary</jats:title><jats:p>We describe a microscope capable of both light sheet fluorescence microscopy and differential interference contrast microscopy (DICM). The two imaging modes, which to the best of our knowledge have not previously been combined, are complementary: light sheet fluorescence microscopy provides three‐dimensional imaging of fluorescently labelled components of multicellular systems with high speed, large fields of view, and low phototoxicity, whereas differential interference contrast microscopy reveals the unlabelled neighbourhood of tissues, organs, and other structures with high contrast and inherent optical sectioning. Use of a single Nomarski prism for differential interference contrast microscopy and a shared detection path for both imaging modes enables simple integration of the two techniques in one custom microscope. We provide several examples of the utility of the resulting instrument, focusing especially on the digestive tract of the larval zebrafish, revealing in this complex and heterogeneous environment anatomical features, the behaviour of commensal microbes, immune cell motions, and more.</jats:p>","journal":"Journal of Microscopy","year":2015,"id":26185,"datarank":1.1605756315718465,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.696919263568099,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.696919263568099,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":13,"citers_with_citation_signal":12,"citers_with_endowment":12,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":152064,"name":"M.J. TAORMINA","orcid":null,"position":1,"is_corresponding":false},{"id":152065,"name":"M. JEMIELITA","orcid":null,"position":2,"is_corresponding":false},{"id":152066,"name":"R. PARTHASARATHY","orcid":null,"position":3,"is_corresponding":false},{"id":152063,"name":"R.P. BAKER","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25611324","pmcid":"PMC4401621","openalex_id":"https://openalex.org/W2096272563","authors":[],"funders":[{"funder_name":"National Science Foundation","grant_id":"0922951","title":"MRI: Development of an Improved Scanned Light Sheet Microscope for Rapid, High-Volume, Three-Dimensional Fluorescence and Dark-Field Microscopies"},{"funder_name":"National Institute of General Medical Sciences of the National Institutes of Health","grant_id":"1P50 GM098911","title":null},{"funder_name":"NICHD NIH HHS","grant_id":"P01 HD022486","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"P50 GM098911","title":null},{"funder_name":"National Institutes of Health","grant_id":"5P50GM098911-05","title":"Microbial Ecology and Theory of Animals Center for Excellence in Systems Biology"}],"total_grants":5,"fwci":5.7213,"citation_percentile":0.95350322,"influential_citations":0,"citation_trend":[{"year":2016,"count":7},{"year":2017,"count":3},{"year":2018,"count":4},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":3}],"oa_status":"closed","license":"Wiley Online Library User Agreement","oa_locations":[{"url":"https://europepmc.org/articles/pmc4401621?pdf=render","host_type":"GREEN"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjmi.12220","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/jmi.12220","host_type":"publisher"},{"url":"https://doi.org/10.1111/jmi.12220","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25611324","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4401621","host_type":"repository"},{"url":"https://dx.doi.org/10.1111/jmi.12220","host_type":""}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Zebrafish Biomedical Research Applications","Cell Image Analysis Techniques","Materials Science","Medicine","Biology","Engineering","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Imaging, Three-Dimensional","Intravital Microscopy","Light","Microscopy, Fluorescence","Microscopy, Interference","Zebrafish"],"mesh_terms":["Intravital Microscopy","Animals","Light","Microscopy, Fluorescence","Microscopy, Interference","Zebrafish","Imaging, Three-Dimensional"],"keywords":["Differential interference contrast microscopy","Light sheet fluorescence microscopy","Microscopy","Fluorescence microscope","Interference microscopy","Microscope","Optical microscope","Optical sectioning","Bright-field microscopy","Optics","Two-photon excitation microscopy","Contrast (vision)","Materials science","Fluorescence","Physics","Scanning electron microscope","Zebrafish","Light Sheet Microscopy","Selective Plane Illumination Microscopy","Nomarski","Imaging, Three-Dimensional","Intravital Microscopy","Light","Microscopy, Fluorescence","Animals","Microscopy, Interference"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T12:25:44.347582Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}