{"doi":"10.1111/jdi.13432","title":"Placental maternally expressed gene 3 differentially methylated region methylation profile is associated with maternal glucose concentration and newborn birthweight","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Aims/Introduction</jats:title><jats:p>Emerging evidence shows that epigenetic modifications occurring during fetal development in response to intrauterine exposures could be one of the mechanisms involved in the early determinants of adult metabolic disorders. This study aimed to investigate whether the placental maternally expressed gene 3 (<jats:italic>MEG3</jats:italic>) deoxyribonucleic acid (DNA) methylation profile is associated with maternal gestational diabetes mellitus status and newborn birthweight.</jats:p></jats:sec><jats:sec><jats:title>Materials and Methods</jats:title><jats:p>Samples for measurement were collected from 23 women with gestational diabetes mellitus and 23 healthy controls. <jats:italic>MEG3</jats:italic> gene expression and DNA methylation levels were assessed using quantitative real‐time polymerase chain reaction and MethylTargetTM, respectively. Pearson correlation analyses were used to examine associations between placental DNA methylation levels and clinical variables of interest. The associated results were adjusted by multivariate linear regression for maternal age, body mass index, height, gestational age and newborn sex as confounders.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>We found that the DNA methylation levels in the <jats:italic>MEG3</jats:italic> differentially methylated region were significantly different between the gestational diabetes mellitus and control groups on the maternal side of the placenta (40.64 ± 2.15 vs 38.33 ± 2.92; <jats:italic>P</jats:italic> = 0.004). Furthermore, the mean <jats:italic>MEG3</jats:italic> DNA methylation levels were correlated positively with maternal fasting glucose concentrations (<jats:italic>R</jats:italic> = 0.603, <jats:italic>P</jats:italic> &lt; 0.001) and newborn birthweight (<jats:italic>R</jats:italic> = 0.568, <jats:italic>P</jats:italic> &lt; 0.001).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The placental DNA methylation status in the <jats:italic>MEG3</jats:italic> differentially methylated region was correlated with maternal glucose concentrations and newborn birthweight. These epigenetic adaptations might contribute to late‐onset obesity, underlining the adverse intrauterine environment.</jats:p></jats:sec>","journal":"Journal of Diabetes Investigation","year":2021,"id":609088,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":527384,"name":"Ying Jiang","orcid":"0000-0001-5760-281X","position":1,"is_corresponding":false},{"id":992653,"name":"Ting Yan","orcid":"0000-0001-9830-6646","position":2,"is_corresponding":false},{"id":831148,"name":"Yuan Chen","orcid":"0000-0002-1572-7232","position":3,"is_corresponding":false},{"id":1565185,"name":"Mengmeng Yang","orcid":null,"position":4,"is_corresponding":false},{"id":1565186,"name":"Min Lv","orcid":null,"position":5,"is_corresponding":false},{"id":1565187,"name":"Fangfang Xi","orcid":null,"position":6,"is_corresponding":false},{"id":1565188,"name":"Juefei Lu","orcid":null,"position":7,"is_corresponding":false},{"id":1565189,"name":"Baihui Zhao","orcid":null,"position":8,"is_corresponding":false},{"id":260982,"name":"Qiong Luo","orcid":"0000-0002-0782-4462","position":9,"is_corresponding":false},{"id":787016,"name":"Cheng Chen","orcid":"0000-0002-2495-7435","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Placental maternally expressed gene 3 differentially methylated region methylation profile is associated with maternal glucose concentration and newborn birthweight","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Aims/Introduction</jats:title><jats:p>Emerging evidence shows that epigenetic modifications occurring during fetal development in response to intrauterine exposures could be one of the mechanisms involved in the early determinants of adult metabolic disorders. This study aimed to investigate whether the placental maternally expressed gene 3 (<jats:italic>MEG3</jats:italic>) deoxyribonucleic acid (DNA) methylation profile is associated with maternal gestational diabetes mellitus status and newborn birthweight.</jats:p></jats:sec><jats:sec><jats:title>Materials and Methods</jats:title><jats:p>Samples for measurement were collected from 23 women with gestational diabetes mellitus and 23 healthy controls. <jats:italic>MEG3</jats:italic> gene expression and DNA methylation levels were assessed using quantitative real‐time polymerase chain reaction and MethylTargetTM, respectively. Pearson correlation analyses were used to examine associations between placental DNA methylation levels and clinical variables of interest. The associated results were adjusted by multivariate linear regression for maternal age, body mass index, height, gestational age and newborn sex as confounders.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>We found that the DNA methylation levels in the <jats:italic>MEG3</jats:italic> differentially methylated region were significantly different between the gestational diabetes mellitus and control groups on the maternal side of the placenta (40.64 ± 2.15 vs 38.33 ± 2.92; <jats:italic>P</jats:italic> = 0.004). Furthermore, the mean <jats:italic>MEG3</jats:italic> DNA methylation levels were correlated positively with maternal fasting glucose concentrations (<jats:italic>R</jats:italic> = 0.603, <jats:italic>P</jats:italic> &lt; 0.001) and newborn birthweight (<jats:italic>R</jats:italic> = 0.568, <jats:italic>P</jats:italic> &lt; 0.001).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The placental DNA methylation status in the <jats:italic>MEG3</jats:italic> differentially methylated region was correlated with maternal glucose concentrations and newborn birthweight. These epigenetic adaptations might contribute to late‐onset obesity, underlining the adverse intrauterine environment.</jats:p></jats:sec>","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33090678","pmcid":"PMC8169366","openalex_id":"https://openalex.org/W3092077766","authors":[],"funders":[{"funder_name":"Medical Science and Technology Project of Zhejiang Province","grant_id":"2018244300","title":null},{"funder_name":"Natural Science Foundation of Zhejiang Province","grant_id":"LQ20H040008","title":null},{"funder_name":"Natural Science Foundation of Zhejiang Province","grant_id":"LY20H040009","title":null}],"total_grants":3,"fwci":0.9198,"citation_percentile":0.80863006,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":6},{"year":2024,"count":4},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jdi.13432","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jdi.13432","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/jdi.13432","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jdi.13432","host_type":"publisher"},{"url":"https://doi.org/10.1111/jdi.13432","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33090678","host_type":"repository"},{"url":"https://doaj.org/article/9ed98def3e37492d895c244b47f8451b","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8169366","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8169366","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8169366?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Gestational Diabetes Research and Management","Epigenetics and DNA Methylation","Birth, Development, and Health","Adult","Birth Weight","Blood Glucose","Case-Control Studies","DNA Methylation","Diabetes, Gestational","Epigenesis, Genetic","Female","Humans","Infant, Newborn","Linear Models","Male","Pregnancy","Pregnancy-Associated Plasma Protein-A","Real-Time Polymerase Chain Reaction"],"mesh_terms":["Adult","Birth Weight","Blood Glucose","Female","Humans","Infant, Newborn","Male","Pregnancy","Pregnancy-Associated Plasma Protein-A","Linear Models","Case-Control Studies","Diabetes, Gestational","DNA Methylation","Epigenesis, Genetic","Real-Time Polymerase Chain Reaction"],"keywords":["DNA methylation","Gestational diabetes","Epigenetics","Medicine","Methylation","Body mass index","Endocrinology","Diabetes mellitus","Placenta","Confounding","MEG3","Differentially methylated regions","Internal medicine","Gestational age","Pregnancy","Andrology","Gestation","Fetus","Gene expression","Biology","Gene","Genetics","Downregulation and upregulation","Gestational Diabetes Mellitus","Maternally Expressed Gene 3 Differentially Methylated Region"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T03:02:50.840202Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}