{"doi":"10.1111/jcpe.70084","title":"Dietary Nitrate Intake and <scp>16S rRNA</scp> ‐Inferred Nitrite‐Generating Capacity of the Subgingival Microbiome May Influence Glucose Metabolism: Results From the Oral Infections Glucose Intolerance and Insulin Resistance Study ( <scp>ORIGINS</scp> )","abstract":"AIMS: To investigate whether the association between the nitrite-generating capacity of the subgingival microbiome and early cardiometabolic risk biomarkers varies by dietary nitrate intake. MATERIALS AND METHODS: Cross-sectional data from 668 participants (mean age 31 ± 9 years, 73% women) were analysed. Dietary nitrate intake was calculated from food frequency questionnaires. Subgingival 16S rRNA sequencing (Illumina, MiSeq) and PICRUSt2 estimated microbial genes. The Microbiome-Induced Nitric Oxide Enrichment Score (MINES) was calculated as a ratio of microbial gene abundances representing enhanced net capacity for NO generation. Adjusted multivariable linear models regressed cardiometabolic risk biomarkers (HbA1c, glucose, insulin, insulin resistance (HOMA-IR), blood pressure) on nitrate intake and MINES together with a MINES × nitrate intake interaction term. RESULTS: Mean nitrate intake was 190 ± 171 mg/day. Significant interactions of MINES and nitrate intake were observed for insulin and HOMA-IR (p < 0.05). Among participants with a low MINES, higher nitrate intake was associated with lower HOMA-IR (1.2 [1.1-1.4] vs. 1.5 [1.3-1.6]; p = 0.002), but levels were similar in those with high MINES (p = 0.84). CONCLUSIONS: A biomarker of higher microbial NO-generating capacity in subgingival plaque is associated with lower insulin and insulin resistance among individuals with lower dietary nitrate intake. Future trials evaluating the cardiometabolic benefits of nitrate-rich diets should incorporate measures of the entire oral microbiome.","journal":"Journal Of Clinical Periodontology","year":2025,"id":549750,"datarank":0.11116181660377697,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.00718973951978516,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.00718973951978516,"corpus_percentile":27.825481550243676,"corpus_rank":9331,"citation_count":1,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.6682,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":271770,"name":"Bruno Bohn","orcid":"0000-0001-8961-6968","position":1,"is_corresponding":false},{"id":246242,"name":"Jeanine M. Genkinger","orcid":"0000-0002-8438-0412","position":2,"is_corresponding":false},{"id":747340,"name":"Rebecca Molinsky","orcid":"0000-0001-9564-0147","position":3,"is_corresponding":false},{"id":461691,"name":"Sumith Roy","orcid":"0000-0002-8887-9835","position":4,"is_corresponding":false},{"id":1444962,"name":"B. J. Paster","orcid":null,"position":5,"is_corresponding":false},{"id":1262558,"name":"Ching‐Yuan Chen","orcid":null,"position":6,"is_corresponding":false},{"id":755826,"name":"Stephen Johnson","orcid":"0000-0003-0685-9863","position":7,"is_corresponding":false},{"id":1259119,"name":"Melana Yuzefpolskaya","orcid":null,"position":8,"is_corresponding":false},{"id":271781,"name":"P.C. Colombo","orcid":"0000-0003-4977-3285","position":9,"is_corresponding":false},{"id":2963,"name":"Michael Rosenbaum","orcid":"0000-0002-8908-9448","position":10,"is_corresponding":false},{"id":3554,"name":"Rob Knight","orcid":"0000-0002-0975-9019","position":11,"is_corresponding":false},{"id":1444963,"name":"Moïse Desvarieux","orcid":null,"position":12,"is_corresponding":false},{"id":245678,"name":"Panos N. Papapanou","orcid":"0000-0002-6538-3618","position":13,"is_corresponding":false},{"id":65159,"name":"DAVID R. JACOBS","orcid":"0000-0002-7232-0543","position":14,"is_corresponding":false},{"id":36326,"name":"Ryan T. Demmer","orcid":"0000-0003-3429-0379","position":15,"is_corresponding":false},{"id":1444961,"name":"Charlene En Hui Goh","orcid":null,"position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T02:54:12.321988Z","pmid":"41448605","pmcid":"PMC13334686","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}