{"doi":"10.1111/jcpe.13715","title":"Downregulation of <scp>miRNA</scp>‐26 in chronic periodontitis interferes with innate immune responses and cell migration by targeting phospholipase <scp>C</scp> beta 1","abstract":"AIM: To evaluate the potential role of miR-26 family members in periodontal pathogenesis by assessing innate immune responses to periopathic bacteria and regulation of cytoskeletal organization. MATERIALS AND METHODS: Expression of miR-26a-5p and miR-26b-5p was quantified in gingival biopsies derived from healthy and periodontally diseased subjects before and after non-surgical (scaling and root planing) therapy by RT-qPCR. Global pathway analysis and luciferase assays were performed for target identification and validation. Cytokine expression was assessed in miR-26a-5p transfected human oral keratinocytes upon stimulation with either live Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans or Pg lipopolysaccharide (LPS). Wound closure assays were performed in cells transfected with miR-26a-5p, while the impact on cytoskeletal organization was assessed by F-actin staining. RESULTS: miR-26a-5p and miR-26b-5p were downregulated in diseased gingiva and restored 4-6 weeks post-therapy to levels comparable with healthy subjects. Target validation assays identified phospholipase C beta 1 as a bona fide novel target exhibiting antagonistic expression pattern in disease and post-therapy cohorts. miR-26a-5p transfected cells secreted higher levels of cytokine/chemokines upon stimulation with periopathogens and demonstrated impaired cell migration and cytoskeletal rearrangement. CONCLUSIONS: Downregulated miR-26a-5p levels in periodontal inflammation may interfere with key cellular functions that may have significant implications for host defence and wound healing.","journal":"Journal Of Clinical Periodontology","year":2022,"id":252000,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9611,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":344186,"name":"Afsar R. Naqvi","orcid":"0000-0001-7436-3056","position":1,"is_corresponding":false},{"id":896504,"name":"Araceli Maria Valverde Estepa","orcid":null,"position":2,"is_corresponding":false},{"id":895921,"name":"Varun Kulkarni","orcid":"0000-0003-4269-9609","position":3,"is_corresponding":false},{"id":896505,"name":"Maria F. Brambila","orcid":null,"position":4,"is_corresponding":false},{"id":895922,"name":"Gloria Martínez-Sandoval","orcid":"0000-0001-5167-2323","position":5,"is_corresponding":false},{"id":896506,"name":"Gabriela Chapa","orcid":null,"position":6,"is_corresponding":false},{"id":895923,"name":"Christine D. Wu","orcid":"0000-0003-0930-7108","position":7,"is_corresponding":false},{"id":895924,"name":"Wei Li","orcid":"0000-0003-2133-2576","position":8,"is_corresponding":false},{"id":424136,"name":"Sona Rivas‐Tumanyan","orcid":"0000-0002-3773-7028","position":9,"is_corresponding":false},{"id":421533,"name":"Salvador Nares","orcid":"0000-0002-2194-1374","position":10,"is_corresponding":false},{"id":895920,"name":"Juhi Raju Uttamani","orcid":"0000-0001-7660-0430","position":0,"is_corresponding":true}],"reference_count":26,"raw_metadata":null,"created_at":"2026-07-19T00:24:46.226372Z","pmid":"36054706","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}