{"doi":"10.1111/jam.15812","title":"Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model","abstract":"Abstract Aims Effects of H2O2 producing electrochemical-bandages (e-bandages) on methicillin-resistant Staphylococcus aureus colonization and biofilm removal were assessed using a porcine explant biofilm model. Transport of H2O2 produced from the e-bandage into explant tissue and associated potential toxicity were evaluated. Methods and Results Viable prokaryotic cells from infected explants were quantified after 48 h treatment with e-bandages in three ex vivo S. aureus infection models: (1) reducing colonization, (2) removing young biofilms and (3) removing mature biofilms. H2O2 concentration-depth profiles in explants/biofilms were measured using microelectrodes. Reductions in eukaryotic cell viability of polarized and nonpolarized noninfected explants were compared. e-Bandages effectively reduced S. aureus colonization (p = 0.029) and reduced the viable prokaryotic cell concentrations of young biofilms (p = 0.029) with limited effects on mature biofilms (p > 0.1). H2O2 penetrated biofilms and explants and reduced eukaryotic cell viability by 32–44% compared to nonpolarized explants. Conclusions H2O2 producing e-bandages were most active when used to reduce colonization and remove young biofilms rather than to remove mature biofilms. Significance and Impact of Study The described e-bandages reduced S. aureus colonization and young S. aureus biofilms in a porcine explant wound model, supporting their further development as an antibiotic-free alternative for managing biofilm infections.","journal":"Journal of Applied Microbiology","year":2022,"id":263469,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9647,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":418595,"name":"Abdelrhman Mohamed","orcid":"0000-0003-2132-0487","position":1,"is_corresponding":false},{"id":911904,"name":"Suzanne Gelston","orcid":null,"position":2,"is_corresponding":false},{"id":670797,"name":"Laure Flurin","orcid":"0000-0001-5687-1081","position":3,"is_corresponding":false},{"id":418594,"name":"Yash S. Raval","orcid":"0000-0003-1161-4000","position":4,"is_corresponding":false},{"id":418597,"name":"Kerryl E. Greenwood‐Quaintance","orcid":"0000-0003-3760-4310","position":5,"is_corresponding":false},{"id":232756,"name":"Robin Patel","orcid":"0000-0001-6344-4141","position":6,"is_corresponding":false},{"id":418598,"name":"Haluk Beyenal","orcid":"0000-0003-3931-0244","position":7,"is_corresponding":false},{"id":520894,"name":"Gretchen Tibbits","orcid":"0000-0002-4285-2258","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T00:26:33.413850Z","pmid":"36073322","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}