{"doi":"10.1111/j.1751-1097.1982.tb04381.x","title":"<i>IN VIVO</i> EXCISION OF PYRIMIDINE DIMERS IS MEDIATED BY A DNA N‐GLYCOSYLASE IN <i>MICROCOCCUS LUTEUS</i> BUT NOT IN HUMAN FIBROBLASTS","abstract":"<jats:p><jats:bold>Abstract</jats:bold> It has been previously shown that <jats:italic>Micrococcus luteus</jats:italic> possesses a pyrimidine dimer‐specific endonuclease which <jats:italic>in vitro</jats:italic>, functions as both an endonuclease and DNA‐glycosylase. To determine if these combined activities function <jats:italic>in vivo</jats:italic>, we have isolated and examined the excision products of UV‐irradiated <jats:italic>M. luteus</jats:italic>. In addition, we have devised a procedure to isolate and examine the excision products from UV‐irradiated human fibroblasts to determine if an endonuclease/glycosylase activity functions in the excision of UV‐induced pyrimidine dimers in human fibroblasts. We find that, <jats:italic>in vivo</jats:italic>, an endonuclease/glycosylase mechanism is utilized extensively in the repair of pyrimidine dimers by <jats:italic>M. luteus</jats:italic>, but that human fibroblasts do not appear to use this mechanism.</jats:p>","journal":"Photochemistry and 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