{"doi":"10.1111/j.1742-4658.2009.07265.x","title":"Biochemical analysis of the human EVL domains in homologous recombination","abstract":"<jats:p>EVL is a member of the ENA/VASP family, which is involved in actin‐remodeling processes. Previously, we reported that human EVL directly interacts with RAD51, which is an essential protein in the homologous recombinational repair of DNA double‐strand breaks, and stimulates RAD51‐mediated recombination reactions <jats:italic>in vitro</jats:italic>. To identify the EVL domain required for the recombination function, we purified the EVL fragments EVL(1–221) and EVL(222–418), which contain the EVH1 and Pro‐rich domains and the EVH2 domain, respectively. We found that EVL(222–418) possesses DNA‐binding and RAD51‐binding activities, and also stimulates RAD51‐mediated homologous pairing. In contrast, EVL(1–221) did not exhibit any of these activities. Therefore, the EVH2 domain, which is highly conserved among the ENA/VASP family proteins, may be responsible for the recombination function of EVL.</jats:p><jats:p><jats:bold>Structured digital abstract</jats:bold></jats:p><jats:p><jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p> <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://mint.bio.uniroma2.it/mint/search/interaction.do?interactionAc=MINT-7239394\">MINT‐7239394</jats:ext-link>: <jats:italic>EVL</jats:italic> (uniprotkb:<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://www.ebi.uniprot.org/entry/Q9UI08\">Q9UI08</jats:ext-link>) <jats:italic>binds</jats:italic> (<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0407\">MI:0407</jats:ext-link>) to <jats:italic>RAD51</jats:italic> (uniprotkb:<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://www.ebi.uniprot.org/entry/Q06609\">Q06609</jats:ext-link>) by <jats:italic>pull down</jats:italic> (<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0096\">MI:0096</jats:ext-link>)</jats:p></jats:list-item>\n</jats:list></jats:p>","journal":"The FEBS 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