{"doi":"10.1111/j.1600-0854.2011.01177.x","title":"Recombinant Heptameric Coatomer Complexes: Novel Tools to Study Isoform‐Specific Functions","abstract":"<jats:p>\n                    <jats:bold>\n                      COPI (coat protein I)‐coated vesicles are implicated in various transport steps within the early secretory pathway. The major structural component of the COPI coat is the heptameric complex coatomer (CM). Recently, four isoforms of CM were discovered that may help explain various transport steps in which the complex has been reported to be involved. Biochemical studies of COPI vesicles currently use CM purified from animal tissue or cultured cells, a mixture of the isoforms, impeding functional and structural studies of individual complexes. Here we report the cloning into single baculoviruses of all CM subunits including their isoforms and their combination for expression of heptameric CM isoforms in insect cells. We show that all four isoforms of recombinant CM are fully functional in an\n                      <jats:italic>in vitro</jats:italic>\n                      COPI vesicle biogenesis assay. These novel tools enable functional and structural studies on CM isoforms and their subcomplexes and allow studying mutants of CM.\n                    </jats:bold>\n                  </jats:p>","journal":"Traffic","year":2011,"id":31156,"datarank":0.828648238679351,"base_score":3.367295829986474,"endowment":3.367295829986474,"self_citation_contribution":0.5050943744979712,"citation_network_contribution":0.32355386418137977,"self_endowment_contribution":0.5050943744979712,"citer_contribution":0.32355386418137977,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":11,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":167596,"name":"Jeroen R. P. M. Strating","orcid":null,"position":1,"is_corresponding":false},{"id":123377,"name":"Rainer Beck","orcid":null,"position":2,"is_corresponding":false},{"id":167597,"name":"Priska Eckert","orcid":null,"position":3,"is_corresponding":false},{"id":145435,"name":"Vincent Popoff","orcid":null,"position":4,"is_corresponding":false},{"id":167598,"name":"Mathias Haag","orcid":null,"position":5,"is_corresponding":false},{"id":122124,"name":"Andrea Hellwig","orcid":null,"position":6,"is_corresponding":false},{"id":167599,"name":"Imre Berger","orcid":"0000-0001-7518-9045","position":7,"is_corresponding":false},{"id":122089,"name":"Britta Brügger","orcid":null,"position":8,"is_corresponding":false},{"id":120496,"name":"Felix T. Wieland","orcid":"0000-0002-3031-1833","position":9,"is_corresponding":false},{"id":167595,"name":"Monika C. Sahlmüller","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21324056","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0854.2011.01177.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0854.2011.01177.x","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Cell Line","COP-Coated Vesicles","Golgi Apparatus","Animals","Mice","Coatomer Protein","Protein Isoforms","Protein Subunits","Recombinant Proteins","Biological Transport"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T06:37:19.212385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}