{"doi":"10.1111/j.1600-0463.2011.02804.x","title":"Involvement of hGLD-2 in cytoplasmic polyadenylation of human p53 mRNA","abstract":null,"journal":"APMIS","year":2011,"id":590260,"datarank":0.703831773066756,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.33109577559855596,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.33109577559855596,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":11,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1510263,"name":"BODIL NORRILD","orcid":null,"position":1,"is_corresponding":false},{"id":1510262,"name":"JACOB A. GLAHDER","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Involvement of hGLD-2 in cytoplasmic polyadenylation of human p53 mRNA","abstract":"Cytoplasmic polyadenylation is a post-transcriptional mechanism regulating mRNA stability and translation. The human p53 3'-untranslated region (3'-UTR) contains two regions similar to cytoplasmic polyadenylation elements (CPEs) just upstream of the poly(A) hexanucleotide. Evaluation of the p53 CPE-like elements was performed by luciferase reporter assays, qPCR, and poly(A) assays. Herein, we report the down regulation of a luciferase reporter fused to the p53 3'-UTR, when human CPE-binding protein 1 (hCPEB1) is overexpressed. This inhibition is partially rescued when hCPEB1fused to hGLD-2 [a human cytoplasmic poly(A) polymerase] is overexpressed instead. The stability of a luciferase mRNA containing the p53 3'-UTR downstream, is decreased when hCPEB1 is overexpressed as seen by qPCR. Expression of hGLD-2 restores the mRNA stability. This is due to elongation of the poly(A) tail as seen by a PCR-based poly(A) test and in vitro poly(A) assay. Taken together, our results suggest that hCPEB1 and hGLD-2 are antagonizing factors regulating p53 mRNA stability.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21995630","pmcid":null,"openalex_id":"https://openalex.org/W1808020867","authors":[],"funders":[],"total_grants":0,"fwci":0.7034,"citation_percentile":0.70533756,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2014,"count":3},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2021,"count":1}],"oa_status":"closed","license":"http://doi.wiley.com/10.1002/tdm_license_1.1","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0463.2011.02804.x","host_type":"publisher"},{"url":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1600-0463.2011.02804.x/fullpdf","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1600-0463.2011.02804.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21995630","host_type":"repository"},{"url":"https://curis.ku.dk/portal/da/publications/involvement-of-hgld2-in-cytoplasmic-polyadenylation-of-human-p53-mrna(04c0c2c8-991e-4d34-8dc6-1adad2568f74).html","host_type":"repository"},{"url":"https://researchprofiles.ku.dk/da/publications/04c0c2c8-991e-4d34-8dc6-1adad2568f74","host_type":"repository"}],"fields_of_study":["Cancer-related Molecular Pathways","RNA Research and Splicing","RNA modifications and cancer"],"mesh_terms":["Cytoplasm","Humans","Polynucleotide Adenylyltransferase","RNA, Messenger","Transcription Factors","Tumor Suppressor Protein p53","3' Untranslated Regions","RNA Stability","Polyadenylation","mRNA Cleavage and Polyadenylation Factors"],"keywords":["Polyadenylation","Three prime untranslated region","Messenger RNA","Luciferase","Molecular biology","Untranslated region","Cytoplasm","Translation (biology)","Biology","Chemistry","Cell biology","Transfection","Gene","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T15:13:42.634975Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}