{"doi":"10.1111/j.1471-4159.1969.tb08993.x","title":"CARBOXYMETHYLATION OF SULPHYDRYL GROUPS IN PROTEOLIPIDS<sup>1</sup>","abstract":"<jats:title>Abstract—</jats:title><jats:p>(1) The sulphydryl groups of brain white matter proteolipids were studied by alkylation with iodoacetic acid and iodoacetamide in an organic solvent medium. To make sterically hindered sulphydryl groups available, the reaction was also carried out in the presence of sodium dodecyl sulphate.</jats:p><jats:p>(2) In all cases, iodoacetamide was a better alkylating agent than was iodoacetic acid.</jats:p><jats:p>(3) Only minimal alkylation of crude white matter proteolipids was obtained in the absence of detergent; addition of sodium dodecyl sulphate increased the availablity of SH groups.</jats:p><jats:p>(4) Purified proteolipids prepared by column chromatography were alkylated to a lesser degree than were crude proteolipids.</jats:p><jats:p>(5) Prior reduction with mercaptoethanol resulted in the quantitative conversion of cysteine to S‐carboxymethylcysteine with either alkylating agent and in both preparations.</jats:p><jats:p>(6) The possibility of a conformational difference between the protein in the crude and purified preparations is discussed.</jats:p>","journal":"Journal of Neurochemistry","year":1969,"id":35498,"datarank":3.5812465638211033,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"self_citation_contribution":0.5289540786924243,"citation_network_contribution":3.052292485128679,"self_endowment_contribution":0.5289540786924243,"citer_contribution":3.052292485128679,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":32,"citers_with_citation_signal":31,"citers_with_endowment":31,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":44.94283201407212,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":181667,"name":"J. 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