{"doi":"10.1111/j.1462-2920.2010.02407.x","title":"Extracellular polymeric substances from\n                    <i>Shewanella</i>\n                    sp. HRCR‐1 biofilms: characterization by infrared spectroscopy and proteomics","abstract":"<jats:title>Summary</jats:title>\n                  <jats:p>\n                    The composition of extracellular polymeric substances (EPS) from\n                    <jats:italic>Shewanella</jats:italic>\n                    sp. HRCR‐1 biofilms was investigated using infrared spectroscopy and proteomics to provide insight into potential ecophysiological functions and redox activity of the EPS. Both bound and loosely associated EPS were extracted from\n                    <jats:italic>Shewanella</jats:italic>\n                    sp. HRCR‐1 biofilms prepared using a hollow‐fibre membrane biofilm reactor. Fourier transform infrared spectra revealed the presence of proteins, polysaccharides, nucleic acids, membrane lipids and fatty acids in the EPS fractions. Using a global proteomic approach, a total of 58 extracellular and outer membrane proteins were identified in the EPS. These included homologues of multiple\n                    <jats:italic>Shewanella oneidensis</jats:italic>\n                    MR‐1 proteins that potentially contribute to key physiological biofilm processes, such as biofilm‐promoting protein BpfA, surface‐associated serine protease, nucleotidases (CpdB and UshA), an extracellular lipase, and oligopeptidases (PtrB and a M13 family oligopeptidase lipoprotein). In addition, 20 redox proteins were found in extracted EPS. Among the detected redox proteins were the homologues of two\n                    <jats:italic>S. oneidensis</jats:italic>\n                    MR‐1\n                    <jats:italic>c</jats:italic>\n                    ‐type cytochromes, MtrC and OmcA, which have been implicated in extracellular electron transfer. Given their detection in the EPS of\n                    <jats:italic>Shewanella</jats:italic>\n                    sp. HRCR‐1 biofilms,\n                    <jats:italic>c</jats:italic>\n                    ‐type cytochromes may contribute to the possible redox activity of the biofilm matrix and play important roles in extracellular electron transfer reactions.\n                  </jats:p>","journal":"Environmental Microbiology","year":2011,"id":684164,"datarank":0.8638352660815922,"base_score":5.75890177387728,"endowment":5.75890177387728,"self_citation_contribution":0.8638352660815922,"citation_network_contribution":0.0,"self_endowment_contribution":0.8638352660815922,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":316,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":769553,"name":"Liang Shi","orcid":"0009-0000-9399-9279","position":1,"is_corresponding":false},{"id":1787350,"name":"Roslyn N. Brown","orcid":null,"position":2,"is_corresponding":false},{"id":1469170,"name":"Yijia Xiong","orcid":null,"position":3,"is_corresponding":false},{"id":1787353,"name":"Jim K. Fredrickson","orcid":null,"position":4,"is_corresponding":false},{"id":1784456,"name":"Margaret F. Romine","orcid":null,"position":5,"is_corresponding":false},{"id":1787355,"name":"Matthew J. Marshall","orcid":null,"position":6,"is_corresponding":false},{"id":1518292,"name":"Mary S. Lipton","orcid":null,"position":7,"is_corresponding":false},{"id":418598,"name":"Haluk Beyenal","orcid":"0000-0003-3931-0244","position":8,"is_corresponding":false},{"id":588711,"name":"Bin Cao","orcid":"0000-0001-8743-2743","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Extracellular polymeric substances from\n                    <i>Shewanella</i>\n                    sp. HRCR‐1 biofilms: characterization by infrared spectroscopy and proteomics","abstract":"<jats:title>Summary</jats:title>\n                  <jats:p>\n                    The composition of extracellular polymeric substances (EPS) from\n                    <jats:italic>Shewanella</jats:italic>\n                    sp. HRCR‐1 biofilms was investigated using infrared spectroscopy and proteomics to provide insight into potential ecophysiological functions and redox activity of the EPS. Both bound and loosely associated EPS were extracted from\n                    <jats:italic>Shewanella</jats:italic>\n                    sp. HRCR‐1 biofilms prepared using a hollow‐fibre membrane biofilm reactor. Fourier transform infrared spectra revealed the presence of proteins, polysaccharides, nucleic acids, membrane lipids and fatty acids in the EPS fractions. Using a global proteomic approach, a total of 58 extracellular and outer membrane proteins were identified in the EPS. These included homologues of multiple\n                    <jats:italic>Shewanella oneidensis</jats:italic>\n                    MR‐1 proteins that potentially contribute to key physiological biofilm processes, such as biofilm‐promoting protein BpfA, surface‐associated serine protease, nucleotidases (CpdB and UshA), an extracellular lipase, and oligopeptidases (PtrB and a M13 family oligopeptidase lipoprotein). In addition, 20 redox proteins were found in extracted EPS. Among the detected redox proteins were the homologues of two\n                    <jats:italic>S. oneidensis</jats:italic>\n                    MR‐1\n                    <jats:italic>c</jats:italic>\n                    ‐type cytochromes, MtrC and OmcA, which have been implicated in extracellular electron transfer. Given their detection in the EPS of\n                    <jats:italic>Shewanella</jats:italic>\n                    sp. HRCR‐1 biofilms,\n                    <jats:italic>c</jats:italic>\n                    ‐type cytochromes may contribute to the possible redox activity of the biofilm matrix and play important roles in extracellular electron transfer reactions.\n                  </jats:p>","is_dataset_classified":null,"base_score":5.75890177387728,"endowment":5.75890177387728,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21251176","pmcid":null,"openalex_id":"https://openalex.org/W1606869502","authors":[],"funders":[],"total_grants":0,"fwci":9.3776,"citation_percentile":0.98780686,"influential_citations":0,"citation_trend":[{"year":2012,"count":19},{"year":2013,"count":18},{"year":2014,"count":22},{"year":2015,"count":16},{"year":2016,"count":22},{"year":2017,"count":22},{"year":2018,"count":17},{"year":2019,"count":18},{"year":2020,"count":23},{"year":2021,"count":33},{"year":2022,"count":27},{"year":2023,"count":18},{"year":2024,"count":24},{"year":2025,"count":20},{"year":2026,"count":11}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2010.02407.x","host_type":"publisher"},{"url":"https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1462-2920.2010.02407.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1462-2920.2010.02407.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21251176","host_type":"repository"}],"fields_of_study":["Advanced Nanomaterials in Catalysis","Microbial Fuel Cells and Bioremediation","Electrochemical sensors and biosensors"],"mesh_terms":["Bacterial Proteins","Chromatography, Liquid","Cytochrome c Group","Electron Transport","Extracellular Space","Membrane Proteins","Oxidation-Reduction","Polymers","Spectroscopy, Fourier Transform Infrared","Biofilms","Bioreactors","Shewanella","Proteomics","Tandem Mass Spectrometry"],"keywords":["Shewanella oneidensis","Biofilm","Extracellular polymeric substance","Shewanella","Biology","Biochemistry","Extracellular","Redox","Polysaccharide","Bacterial outer membrane","Biofouling","Electron transfer","Microbiology","Bacteria","Chemistry","Membrane","Escherichia coli","Gene"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.19498605.v1","title":"Additional file 1 of Effects of cadmium sulfide nanoparticles on sulfate bioreduction and oxidative stress in Desulfovibrio desulfuricans","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.19498605","title":"Additional file 1 of Effects of cadmium sulfide nanoparticles on sulfate bioreduction and oxidative stress in Desulfovibrio desulfuricans","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T13:26:38.161418Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}