{"doi":"10.1111/j.1440-169x.2006.00897.x","title":"Identification and origin of the germline stem cells as revealed by the expression of <i>nanos</i>‐related gene in planarians","abstract":"<jats:p>The planarian's remarkable regenerative ability is thought to be supported by the stem cells (neoblasts) found throughout its body. Here we report the identification of a subpopulation of neoblasts, which was revealed by the expression of the <jats:italic>nanos</jats:italic>‐related gene of the planarian <jats:italic>Dugesia japonica</jats:italic>, termed <jats:italic>Djnos</jats:italic>. <jats:italic>Djnos</jats:italic>‐expressing cells in the asexual planarian were distributed to the prospective ovary or testes forming region in the sexual planarian. During sexualization, <jats:italic>Djnos</jats:italic>‐expressing cells produce germ cells, suggesting that in the asexual state these cells were kept as germline stem cells for the oogonia and spermatogonia. Interestingly, the germline stem cells were indistinguishable from the neoblasts by morphology and X‐ray sensitivity and did not seem to contribute to the regeneration at all. Germline stem cells initially appear in the growing infant planarian, suggesting that germline stem cells are separated from somatic stem cells in the planarian. Thus, planarian neoblasts can be classified into two groups; somatic stem cells for regeneration and tissue renewal, and germline stem cells for production of germ cells during sexualization. However, <jats:italic>Djnos</jats:italic>‐positive cells appeared in the newly formed trunk region from the head piece, suggesting that somatic stem cells can convert to germline stem cells.</jats:p>","journal":"Development, Growth &amp; Differentiation","year":2006,"id":673438,"datarank":0.7357912167657645,"base_score":4.90527477843843,"endowment":4.90527477843843,"self_citation_contribution":0.7357912167657645,"citation_network_contribution":0.0,"self_endowment_contribution":0.7357912167657645,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":134,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1759493,"name":"Norito Shibata","orcid":null,"position":1,"is_corresponding":false},{"id":1759494,"name":"Hidefumi Orii","orcid":null,"position":2,"is_corresponding":false},{"id":1759495,"name":"Reiko Amikura","orcid":null,"position":3,"is_corresponding":false},{"id":1759496,"name":"Takashige Sakurai","orcid":null,"position":4,"is_corresponding":false},{"id":1275142,"name":"Kiyokazu Agata","orcid":"0000-0002-5195-2576","position":5,"is_corresponding":false},{"id":407231,"name":"Satoru Kobayashi","orcid":"0000-0003-4390-5726","position":6,"is_corresponding":false},{"id":1299046,"name":"Kenji Watanabe","orcid":"0000-0002-6026-9066","position":7,"is_corresponding":false},{"id":1526064,"name":"Kimihiro Sato","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification and origin of the germline stem cells as revealed by the expression of <i>nanos</i>‐related gene in planarians","abstract":"<jats:p>The planarian's remarkable regenerative ability is thought to be supported by the stem cells (neoblasts) found throughout its body. Here we report the identification of a subpopulation of neoblasts, which was revealed by the expression of the <jats:italic>nanos</jats:italic>‐related gene of the planarian <jats:italic>Dugesia japonica</jats:italic>, termed <jats:italic>Djnos</jats:italic>. <jats:italic>Djnos</jats:italic>‐expressing cells in the asexual planarian were distributed to the prospective ovary or testes forming region in the sexual planarian. During sexualization, <jats:italic>Djnos</jats:italic>‐expressing cells produce germ cells, suggesting that in the asexual state these cells were kept as germline stem cells for the oogonia and spermatogonia. Interestingly, the germline stem cells were indistinguishable from the neoblasts by morphology and X‐ray sensitivity and did not seem to contribute to the regeneration at all. Germline stem cells initially appear in the growing infant planarian, suggesting that germline stem cells are separated from somatic stem cells in the planarian. Thus, planarian neoblasts can be classified into two groups; somatic stem cells for regeneration and tissue renewal, and germline stem cells for production of germ cells during sexualization. However, <jats:italic>Djnos</jats:italic>‐positive cells appeared in the newly formed trunk region from the head piece, suggesting that somatic stem cells can convert to germline stem cells.</jats:p>","is_dataset_classified":null,"base_score":4.90527477843843,"endowment":4.90527477843843,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17118016","pmcid":null,"openalex_id":"https://openalex.org/W2012157613","authors":[],"funders":[],"total_grants":0,"fwci":3.561,"citation_percentile":0.93160793,"influential_citations":0,"citation_trend":[{"year":2012,"count":14},{"year":2013,"count":6},{"year":2014,"count":9},{"year":2015,"count":5},{"year":2016,"count":7},{"year":2017,"count":5},{"year":2018,"count":9},{"year":2019,"count":5},{"year":2020,"count":2},{"year":2021,"count":9},{"year":2022,"count":6},{"year":2023,"count":4},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1440-169X.2006.00897.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1440-169X.2006.00897.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1440-169x.2006.00897.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17118016","host_type":"repository"}],"fields_of_study":["Planarian Biology and Electrostimulation","Marine Ecology and Invasive Species"],"mesh_terms":["Amino Acid Sequence","Animals","DNA-Binding Proteins","Germ Cells","Molecular Sequence Data","Planarians","Stem Cells","Helminth Proteins","Genes, Helminth","Gene Expression Regulation, Developmental"],"keywords":["Planarian","Germline","Biology","Stem cell","Somatic cell","Cell biology","Adult stem cell","Regeneration (biology)","Embryonic stem cell","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Gender equality"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T13:26:01.864844Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}