{"doi":"10.1111/j.1432-1033.1993.tb18412.x","title":"Gene structure of <i>CYP3A4</i>, an adult‐specific form of cytochrome <i>P</i>450 in human livers, and its transcriptional control","abstract":"<jats:p>CYP3A4 is the adult‐specific form of cytochrome <jats:italic>P</jats:italic>450 in human livers [Komori, M., Nishio, K., Kitada, M., Shiramatsu, K., Muroya, K., Soma, M., Nagashima, K. &amp; Kamataki, T. (1990) <jats:italic>Biochemistry 29</jats:italic>, 4430–4433]. The sequences of three genomic clones for <jats:italic>CYP3A4</jats:italic> were analyzed for all exons, exon‐intron junctions and the 5′‐flanking region from the major transcription site to nucleotide position ‐1105, and compared with those of the <jats:italic>CYP3A7</jats:italic> gene, a fetal‐specific form of cytochrome <jats:italic>P</jats:italic>450 in humans. The results showed that the identity of 5′‐flanking sequences between <jats:italic>CYP3A4</jats:italic> and <jats:italic>CYP3A7</jats:italic> genes was 91%, and that each 5′‐flanking region had characteristic sequences termed as NFSE (<jats:italic>P</jats:italic>450<jats:sub>NF</jats:sub>‐specific element) and HFLaSE (<jats:italic>P</jats:italic>450HFLa specific element), respectively. A basic transcription element (BTE) also lay in the 5′‐flanking region of the <jats:italic>CYP3A4</jats:italic> gene as seen in many <jats:italic>CYP</jats:italic> genes [Yanagida, A., Sogawa, K., Yasumoto, K. &amp; Fujii‐Kuriyama, Y. (1990) <jats:italic>Mol. Cell. Biol. 10</jats:italic>, 1470–1475]. The BTE binding factor (BTEB) was present in both adult and fetal human livers.</jats:p><jats:p>To examine the transcriptional activity of the <jats:italic>CYP3A4</jats:italic> gene, DNA fragments in the 5′‐flanking region of the gene were inserted in front of the simian virus 40 promoter and the chloramphenicol acetyltransferase structural gene, and the constructs were transfected in HepG2 cells. The analysis of the chloramphenicol acetyltransferase activity indicated that (a) specific element(s) which could bind with a factor(s) in livers was present in the 5′‐flanking region of the <jats:italic>CYP3A4</jats:italic> gene to show the transcriptional activity.</jats:p>","journal":"European Journal of Biochemistry","year":1993,"id":22185,"datarank":16.408148321167157,"base_score":5.351858133476067,"endowment":5.351858133476067,"self_citation_contribution":0.8027787200214102,"citation_network_contribution":15.605369601145746,"self_endowment_contribution":0.8027787200214102,"citer_contribution":15.605369601145746,"corpus_percentile":null,"corpus_rank":null,"citation_count":210,"citer_count":200,"citers_with_citation_signal":190,"citers_with_endowment":190,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":140361,"name":"Kenji TOIDE","orcid":null,"position":1,"is_corresponding":false},{"id":140362,"name":"Ryuji KITAMURA","orcid":null,"position":2,"is_corresponding":false},{"id":140363,"name":"Masako FUJITA","orcid":null,"position":3,"is_corresponding":false},{"id":140364,"name":"Sanae TAGAWA","orcid":null,"position":4,"is_corresponding":false},{"id":140365,"name":"Susumu ITOH","orcid":null,"position":5,"is_corresponding":false},{"id":140366,"name":"Tetsuya KAMATAKI","orcid":null,"position":6,"is_corresponding":false},{"id":140360,"name":"Hisashi HASHIMOTO","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.351858133476067,"endowment":5.351858133476067,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8269949","pmcid":null,"openalex_id":"https://openalex.org/W1963627631","authors":[],"funders":[],"total_grants":0,"fwci":3.2507,"citation_percentile":0.91189759,"influential_citations":12,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":7},{"year":2014,"count":5},{"year":2015,"count":6},{"year":2016,"count":3},{"year":2017,"count":3},{"year":2018,"count":3},{"year":2019,"count":5},{"year":2020,"count":4},{"year":2021,"count":3},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1993.tb18412.x","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1993.tb18412.x","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1993.tb18412.x","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1993.tb18412.x","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1993.tb18412.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1432-1033.1993.tb18412.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8269949","host_type":"repository"}],"fields_of_study":["Pharmacogenetics and Drug Metabolism","Drug Transport and Resistance Mechanisms","Microbial Metabolic Engineering and Bioproduction","Biology","Medicine","Adult","Base Sequence","Cells, Cultured","Cytochrome P-450 CYP3A","Cytochrome P-450 Enzyme System","DNA","Gene Expression Regulation, Enzymologic","Humans","Liver","Mixed Function Oxygenases","Molecular Sequence Data","Mutation","Nuclear Proteins","Promoter Regions, Genetic","Sequence Deletion","Transcription, Genetic"],"mesh_terms":["Adult","Base Sequence","Cells, Cultured","Cytochrome P-450 Enzyme System","DNA","Humans","Mixed Function Oxygenases","Liver","Molecular Sequence Data","Mutation","Nuclear Proteins","Promoter Regions, Genetic","Transcription, Genetic","Gene Expression Regulation, Enzymologic","Sequence Deletion","Cytochrome P-450 CYP3A"],"keywords":["Chloramphenicol acetyltransferase","Biology","5' flanking region","Gene","Molecular biology","Exon","Transcription (linguistics)","Intron","Genetics","Regulatory sequence","Reporter gene","Gene expression","Promoter"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T18:12:40.842003Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}