{"doi":"10.1111/j.1432-1033.1993.tb18174.x","title":"Cholesterol trafficking in steroidogenic cells","abstract":"<jats:p>Peptide hormones activate steroid hormone biosynthesis in responsive tissues by stimulating the delivery of cholesterol to a steroidogenic pool, thought to be located in the inner mitochondrial membrane. At this site, it is metabolized to pregnenolone, the precursor of the steroid hormones, by side‐chain‐cleaving cytochrome <jats:italic>P</jats:italic>‐450 (cytochrome <jats:italic>P</jats:italic>‐450scc). In the presence of aminoglutethimide (an inhibitor of cytochrome <jats:italic>P</jats:italic>‐450scc) and an activating stimulus, cholesterol accumulates in the steroidogenic pool, and increased pregnenolone generation is observed upon removal of the inhibitor. Using Y‐1 adrenocortical cells and MA‐10 Leydig tumor cells, we now provide evidence for a distinct, functionally relevant cholesterol pool which precedes the steroidogenic pool, which we designate the pre‐steroidogenic pool. This pool was defined by activating the cells with 8‐bromo‐adenosine 3′,5′‐cyclic monophosphoric acid in the presence of cycloheximide, an inhibitor of steriodogenesis. Following a wash procedure, which removed 8‐bromo‐adenosine 3′,5′‐cyclic monophosphoric acid and cyclohcximide, augmented pregnenolone synthesis was observed. Unlike synthesis from the steroidogenic pool, pregnenolone formation from pre‐steroidogenic pool in Y‐1 cells indicates that this pool is somewhat smaller than the steroidogenic pool. The results support a cholesterol‐trafficking model in which cycloheximide‐sensitive transport from the pre‐steroidogenic pool to the steroidogenic pool precedes metabolism, and is regulated by cAMP.</jats:p>","journal":"European Journal of Biochemistry","year":1993,"id":36643,"datarank":1.480513109815623,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"self_citation_contribution":0.5101796072493234,"citation_network_contribution":0.9703335025662996,"self_endowment_contribution":0.5101796072493234,"citer_contribution":0.9703335025662996,"corpus_percentile":null,"corpus_rank":null,"citation_count":29,"citer_count":29,"citers_with_citation_signal":27,"citers_with_endowment":27,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":185161,"name":"Tingsen XU","orcid":null,"position":1,"is_corresponding":false},{"id":185162,"name":"J. David LAMBETH","orcid":null,"position":2,"is_corresponding":false},{"id":185160,"name":"Victoria L. STEVENS","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8375394","pmcid":null,"openalex_id":"https://openalex.org/W1648866459","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"DK 27373","title":null}],"total_grants":1,"fwci":3.9061,"citation_percentile":0.9330215,"influential_citations":1,"citation_trend":[{"year":2016,"count":1},{"year":2021,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1993.tb18174.x","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1993.tb18174.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1432-1033.1993.tb18174.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8375394","host_type":"repository"}],"fields_of_study":["Cholesterol and Lipid Metabolism","Estrogen and related hormone effects","Drug Transport and Resistance Mechanisms","Biology","Medicine","Chemistry","8-Bromo Cyclic Adenosine Monophosphate","Adrenal Cortex Neoplasms","Aminoglutethimide","Animals","Cholesterol","Cycloheximide","Leydig Cells","Male","Rats","Steroids","Tumor Cells, Cultured"],"mesh_terms":["Adrenal Cortex Neoplasms","Aminoglutethimide","Animals","Cholesterol","Cycloheximide","Leydig Cells","Male","Steroids","Tumor Cells, Cultured","8-Bromo Cyclic Adenosine Monophosphate","Rats"],"keywords":["Pregnenolone","Cholesterol side-chain cleavage enzyme","Cycloheximide","Aminoglutethimide","Steroidogenic acute regulatory protein","Biology","Hormone","Endocrinology","Internal medicine","Chemistry","Metabolism","Biochemistry","Steroid","Cytochrome P450","Protein biosynthesis","Gene expression","Aromatase"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T16:59:33.186485Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}