{"doi":"10.1111/j.1432-1033.1990.tb19372.x","title":"Structural and functional characterization of human aromatase <i>P</i>‐450 gene","abstract":"<jats:p>The gene encoding aromatase <jats:italic>P</jats:italic>‐450 (CYP XIX) has been isolated from two types of human genomic DNA libraries. It spans at least 70 kb and consists of 10 exons. The translational initiation site and the termination site are located in exon 2 and exon 10, respectively. The promoter region of the gene contains a TATA box, a CAAT box and two putative AP‐1 binding sites beginning at –28, –83, –55 and –68 bp, respectively, from the transcriptional initiation site. In addition, a palindromic nucleotide sequence is observed between –209 and –196 and two types of repetitious hexanucleotide (consensus: AATGAA and CCAT<jats:sup>AA</jats:sup><jats:sub>GG</jats:sub>) are also present within the regions between –485 and –433 and between –358 and –331. Transient expression studies of chloramphenicol acetyltransferase constructs bearing various lengths of 5′‐flanking region of the gene show that the region between –500 and –243 contains negative <jats:italic>cis</jats:italic>‐acting element(s), whereas the region between –242 and –183 is required for efficient transcriptional activity. Northern blot analysis demonstrates that the expression of aromatose <jats:italic>P</jats:italic>‐450 gene is remarkably stimulated by treatment of cells with 12‐<jats:italic>O</jats:italic>‐tetradecanoyl‐phorbol 13‐acetate. By chloramphenicol acetyltransferase assay the region up to nucleotide position –242 relative to the transcriptional initiation site is shown to participate in the transcriptional responsiveness to this phorbol ester.</jats:p>","journal":"European Journal of Biochemistry","year":1990,"id":20825,"datarank":7.8311183840518614,"base_score":4.882801922586371,"endowment":4.882801922586371,"self_citation_contribution":0.7324202883879557,"citation_network_contribution":7.098698095663906,"self_endowment_contribution":0.7324202883879557,"citer_contribution":7.098698095663906,"corpus_percentile":null,"corpus_rank":null,"citation_count":131,"citer_count":122,"citers_with_citation_signal":113,"citers_with_endowment":113,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136361,"name":"Masako TERASHIMA","orcid":null,"position":1,"is_corresponding":false},{"id":136362,"name":"Takeshi KAWAMOTO","orcid":null,"position":2,"is_corresponding":false},{"id":136363,"name":"Hidetoshi SUMIMOTO","orcid":null,"position":3,"is_corresponding":false},{"id":136364,"name":"Yuichi YOKOYAMA","orcid":null,"position":4,"is_corresponding":false},{"id":136365,"name":"Isao KURIBAYASHI","orcid":null,"position":5,"is_corresponding":false},{"id":136366,"name":"Yasuhiro MITSUUCHI","orcid":null,"position":6,"is_corresponding":false},{"id":136367,"name":"Tomoho MAEDA","orcid":null,"position":7,"is_corresponding":false},{"id":136368,"name":"Yasutake YAMAMOTO","orcid":null,"position":8,"is_corresponding":false},{"id":136369,"name":"Yusuke SAGARA","orcid":null,"position":9,"is_corresponding":false},{"id":136370,"name":"Hisao IKEDA","orcid":null,"position":10,"is_corresponding":false},{"id":136371,"name":"Yutaka SHIZUTA","orcid":null,"position":11,"is_corresponding":false},{"id":136360,"name":"Katsumi TODA","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.882801922586371,"endowment":4.882801922586371,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2171939","pmcid":null,"openalex_id":"https://openalex.org/W2093997497","authors":[],"funders":[],"total_grants":0,"fwci":5.2879,"citation_percentile":0.95618829,"influential_citations":1,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2017,"count":3},{"year":2019,"count":2},{"year":2023,"count":2}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1990.tb19372.x","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1990.tb19372.x","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1990.tb19372.x","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1990.tb19372.x","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1990.tb19372.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1432-1033.1990.tb19372.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2171939","host_type":"repository"}],"fields_of_study":["Estrogen and related hormone effects","Genomics and Chromatin Dynamics","DNA and Nucleic Acid Chemistry","Medicine","Biology","Amino Acid Sequence","Aromatase","Base Sequence","Blotting, Northern","Chloramphenicol O-Acetyltransferase","Cloning, Molecular","Cosmids","Cyclic AMP","Exons","Humans","Molecular Sequence Data","Promoter Regions, Genetic","Tetradecanoylphorbol Acetate","Transfection","Tumor Cells, Cultured"],"mesh_terms":["Cyclic AMP","Amino Acid Sequence","Aromatase","Base Sequence","Cloning, Molecular","Cosmids","Exons","Humans","Molecular Sequence Data","Promoter Regions, Genetic","Tetradecanoylphorbol Acetate","Transfection","Tumor Cells, Cultured","Blotting, Northern","Chloramphenicol O-Acetyltransferase"],"keywords":["Chloramphenicol acetyltransferase","TATA box","Molecular biology","Exon","Gene","Biology","CAAT box","Promoter","Acetyltransferase","Reporter gene","Northern blot","5' flanking region","Transcriptional regulation","Gene expression","Primer extension","Regulatory sequence","Genetics","Messenger RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T12:06:54.084182Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}