{"doi":"10.1111/j.1432-1033.1971.tb01566.x","title":"An Efficient Procedure for the Isolation of Polyribosomes from Tissue Culture","abstract":"<jats:p>In this paper an efficient procedure is described for the isolation of polysomes from tissue culture cells. Optimal yields, especially of the heavier classes of polysomes can only be obtained at a rather high KC1 concentration in the presence of nonionic detergent. Evidence is provided that the lower polysomal yield after extraction at low salt concentration is not due to breakdown of the polysomes by RNAase.</jats:p><jats:p>Furthermore, the yield of polysomes is pH dependent only at low salt concentration, while at high salt concentration no influence of the pH is observed. Refreshing of growth medium several hours before harvesting the cells, increases the ratio of polysomes to 80 S monomers.</jats:p>","journal":"European Journal of Biochemistry","year":1971,"id":18463,"datarank":5.825268184967294,"base_score":4.060443010546419,"endowment":4.060443010546419,"self_citation_contribution":0.6090664515819629,"citation_network_contribution":5.216201733385331,"self_endowment_contribution":0.6090664515819629,"citer_contribution":5.216201733385331,"corpus_percentile":null,"corpus_rank":null,"citation_count":57,"citer_count":55,"citers_with_citation_signal":51,"citers_with_endowment":51,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":128247,"name":"Ton J. M. Berns","orcid":null,"position":1,"is_corresponding":false},{"id":118083,"name":"Hans Bloemendal","orcid":null,"position":2,"is_corresponding":false},{"id":128246,"name":"Arno L. J. Gielkens","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.060443010546419,"endowment":4.060443010546419,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"5166922","pmcid":null,"openalex_id":"https://openalex.org/W1990338613","authors":[],"funders":[],"total_grants":0,"fwci":5.7355,"citation_percentile":0.96469976,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1971.tb01566.x","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1971.tb01566.x","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1971.tb01566.x","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1971.tb01566.x","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1971.tb01566.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1432-1033.1971.tb01566.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/5166922","host_type":"repository"},{"url":"http://hdl.handle.net/2066/142596","host_type":"repository"}],"fields_of_study":["Biopolymer Synthesis and Applications","Protein purification and stability","Biology","Medicine","Acetates","Animals","Bile Acids and Salts","Cattle","Cell Fractionation","Centrifugation, Density Gradient","Culture Media","Culture Techniques","Detergents","Epithelial Cells","HeLa Cells","Hydrogen-Ion Concentration","Lens, Crystalline","Magnesium","Methods","Mice","Osmolar Concentration","Potassium Chloride","Rauscher Virus","Ribonucleases","Ribosomes","Spleen","Sucrose","Thymus Gland","Trypsin"],"mesh_terms":["Acetates","Animals","Bile Acids and Salts","Cattle","Cell Fractionation","Centrifugation, Density Gradient","Culture Media","Detergents","Epithelial Cells","HeLa Cells","Hydrogen-Ion Concentration","Lens, Crystalline","Magnesium","Methods","Osmolar Concentration","Potassium Chloride","Rauscher Virus","Ribonucleases","Ribosomes","Spleen","Sucrose","Thymus Gland","Trypsin","Culture Techniques","Mice","Hela Cells"],"keywords":["Polysome","Yield (engineering)","Salt (chemistry)","Monomer","Biochemistry","Chemistry","Isolation (microbiology)","Extraction (chemistry)","Tissue culture","Chromatography","Biology","In vitro","Ribosome","RNA","Organic chemistry","Materials science","Bioinformatics","Polymer"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-03T22:08:42.724181Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}