{"doi":"10.1111/j.1365-3083.1995.tb03562.x","title":"Native Human Serum Amyloid P Component is a Single Pentamer","abstract":"<jats:p>Serum amyloid P component (SAP) and C‐reactive protein (CRP) are members of the pentraxin protein family. SAP is the precursor protein to amyloid P component present in all forms of amyloidosis. The prevailing notion is that SAP in circulation has the form of a double pentameric molecule (decamer) whereas CRP is a single pentameric molecule.</jats:p><jats:p>We have investigated by gel permeation chromatography the M<jats:sub>r</jats:sub> of SAP in freshly collected human serum and of SAP purified by carbohydrate affinity chromatography and anion exchange chromatography. SAP was monitored by quantitative immunoelectrophoresis and ELISA. and SAP peak fractions were analysed by use of SDS‐PAGE, Western blotting, and electron microscopy. The results indicate that native SAP circulates as a single pentamer, a part of which forms complexes with C4b‐binding protein. The properties of SAP changed during purification as indicated by rocket immuno‐electrophoresis and electron microscopy. Thus, electron micrographs of purified SAP showed a predominance of decamers. However, the decamer form of SAP reversed to single pentamers when purified SAP was incorporated into SAP‐depleted serum.</jats:p>","journal":"Scandinavian Journal of Immunology","year":1995,"id":35938,"datarank":1.3925909173956075,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.8982153874949581,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.8982153874949581,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":21,"citers_with_citation_signal":19,"citers_with_endowment":19,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":44.94283201407212,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":183036,"name":"O. ANDERSEN","orcid":null,"position":1,"is_corresponding":false},{"id":183037,"name":"E. HOLM NIELSEN","orcid":null,"position":2,"is_corresponding":false},{"id":183038,"name":"S. E. SVEHAG","orcid":null,"position":3,"is_corresponding":false},{"id":183035,"name":"I. J. SØRENSEN","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.295836866004329,"endowment":3.295836866004329,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7871385","pmcid":null,"openalex_id":"https://openalex.org/W2009311541","authors":[],"funders":[],"total_grants":0,"fwci":1.0838,"citation_percentile":0.76394861,"influential_citations":1,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":2},{"year":2020,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3083.1995.tb03562.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3083.1995.tb03562.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1365-3083.1995.tb03562.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7871385","host_type":"repository"},{"url":"https://researchprofiles.ku.dk/da/publications/0fe1e919-f257-4018-ad14-c47db60f10b8","host_type":"repository"}],"fields_of_study":["Amyloidosis: Diagnosis, Treatment, Outcomes","Trace Elements in Health","Protein Interaction Studies and Fluorescence Analysis","Chemistry","Medicine","Biology","Biopolymers","Blotting, Western","Chromatography, Gel","Enzyme-Linked Immunosorbent Assay","Humans","Immunoelectrophoresis","Microscopy, Electron","Serum Amyloid P-Component"],"mesh_terms":["Serum Amyloid P-Component","Biopolymers","Chromatography, Gel","Enzyme-Linked Immunosorbent Assay","Humans","Immunoelectrophoresis","Microscopy, Electron","Blotting, Western"],"keywords":["Pentamer","Serum amyloid P component","Chemistry","Gel permeation chromatography","Affinity chromatography","Amyloid (mycology)","Biochemistry","Immunoelectrophoresis","Polyacrylamide gel electrophoresis","Chromatography","Biology","Antibody","Enzyme","C-reactive 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