{"doi":"10.1111/j.1365-2826.2008.01734.x","title":"Measurement of Immediate‐Early Gene Activation‐ <i>c‐fos</i> and Beyond","abstract":"<jats:p>Immediate‐early genes (IEG) are powerful tools for identifying activated neurosecretory neurones and extended circuits that affect neuroendocrine functions. The generally acknowledged scenario is when cells became activated, IEGs expressed and IEG‐encoded transcription factors affect target gene expression. However, there are several examples in which: (i) neuronal activation occurs without induction of IEGs; (ii) IEG induction is not related to challenge‐induced neuropeptide expression; and (iii) markers of neuronal activation are not expressed in chronically activated neurones. In spite of these limitations, the use of c‐Fos and other regulatory‐ or effector transcription factors as markers of neuronal activation will continue to be an extremely powerful technique. Recently‐developed models, including transgenic mice expressing different marker genes under the regulation of IEG promoters, will help to monitor neuronal activity <jats:italic>in vivo</jats:italic> or <jats:italic>ex vivo</jats:italic> and to reveal connection between activated neurones. Furthermore, combinations between novel imaging techniques, such as magnetic resonance and IEG‐based mapping strategies, will open new means with which to study functional activity in the neurosecretory systems.</jats:p>","journal":"Journal of Neuroendocrinology","year":2008,"id":40720,"datarank":11.168415563155135,"base_score":6.194405391104672,"endowment":6.194405391104672,"self_citation_contribution":0.9291608086657009,"citation_network_contribution":10.239254754489433,"self_endowment_contribution":0.9291608086657009,"citer_contribution":10.239254754489433,"corpus_percentile":null,"corpus_rank":null,"citation_count":489,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":32.561262168512926,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":197349,"name":"K. J. Kovács","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.194405391104672,"endowment":6.194405391104672,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18601687","pmcid":null,"openalex_id":"https://openalex.org/W2061690520","authors":[],"funders":[],"total_grants":0,"fwci":9.8269,"citation_percentile":0.98739277,"influential_citations":24,"citation_trend":[{"year":2012,"count":34},{"year":2013,"count":26},{"year":2014,"count":20},{"year":2015,"count":23},{"year":2016,"count":27},{"year":2017,"count":15},{"year":2018,"count":26},{"year":2019,"count":35},{"year":2020,"count":34},{"year":2021,"count":31},{"year":2022,"count":35},{"year":2023,"count":28},{"year":2024,"count":35},{"year":2025,"count":31},{"year":2026,"count":18}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2826.2008.01734.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2826.2008.01734.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1365-2826.2008.01734.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18601687","host_type":"repository"}],"fields_of_study":["Receptor Mechanisms and Signaling","Adipose Tissue and Metabolism","RNA Interference and Gene Delivery","Biology","Medicine","Animals","Animals, Genetically Modified","Animals, Suckling","Biomarkers","Gene Expression Regulation","Genes, Immediate-Early","Neurons","Neurosecretory Systems","Proto-Oncogene Proteins c-fos","Transcriptional Activation","Tyrosine 3-Monooxygenase"],"mesh_terms":["Animals","Animals, Suckling","Gene Expression Regulation","Neurons","Neurosecretory Systems","Tyrosine 3-Monooxygenase","Biomarkers","Transcriptional Activation","Proto-Oncogene Proteins c-fos","Genes, Immediate-Early","Animals, Genetically Modified"],"keywords":["Immediate early gene","c-Fos","Effector","Neuroscience","Biology","Transcription factor","Premovement neuronal activity","Gene expression","Cell biology","Transgene","Regulation of gene expression","Neuropeptide","Genetically modified mouse","Gene","Receptor","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-12T13:00:28.851493Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":100.0,"fair_a":70.0,"fair_i":0.0,"fair_r":25.0,"fair_zscore":0.0532,"fair_rationale":{"fair_score":48.75,"has_llm":false,"dimensions":{"F":{"name":"Findable","score":100.0,"criteria":[{"key":"f_has_doi","label":"Has a persistent DOI","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"DOI present","rationale":null},{"key":"f_repository_presence","label":"Indexed in repositories / literature DBs","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"datacite=0, pmcid=False, pmid=True","rationale":null},{"key":"f_persistent_ids","label":"Resolvable scholarly identifiers (OpenAlex)","kind":"deterministic","weight":0.5,"fraction":1.0,"signal":"OpenAlex id present","rationale":null}]},"A":{"name":"Accessible","score":70.0,"criteria":[{"key":"a_open_access","label":"Open Access / files deposited","kind":"deterministic","weight":1.5,"fraction":0.5,"signal":"files/OA location present but not flagged OA","rationale":null},{"key":"a_retrievable","label":"Free full text retrievable","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"4 OA location(s)","rationale":null}]},"I":{"name":"Interoperable","score":0.0,"criteria":[{"key":"i_linked_data","label":"Linked datasets / DataCite relations","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"linked_datasets=0, datacite=0","rationale":null},{"key":"i_standard_ids","label":"References data via standard accessions","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"accessions=0, trials=0","rationale":null}]},"R":{"name":"Reusable","score":25.0,"criteria":[{"key":"r_license","label":"Clear, open reuse license","kind":"deterministic","weight":1.5,"fraction":0.5,"signal":"license present (http://onlinelibrary.wiley.com/termsAndConditions#vor)","rationale":null},{"key":"r_downloads","label":"Demonstrated reuse (downloads)","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"downloads=0","rationale":null},{"key":"r_version","label":"Versioned / maintained","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"no version chain","rationale":null},{"key":"r_dataset","label":"Classified as a data resource","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"not a dataset","rationale":null}]}},"suggestions":["Link the underlying datasets via DOIs / DataCite relations.","Reference data using standard accessions (e.g. GEO, PDB, ClinicalTrials.gov).","Maintain explicit versioning for the dataset.","Make the paper/data Open Access or deposit the files in an open repository.","Attach a clear, open reuse license (e.g. CC-BY or CC0)."],"model":null,"agent_version":"fair_agent_v1","fulltext_source":"abstract_only"},"fair_model":null,"fair_agent_version":"fair_agent_v1","fair_fulltext_source":"abstract_only","fair_has_llm":false,"fair_computed_at":"2026-06-12T18:41:23.121204Z","clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}