{"doi":"10.1111/j.1365-2222.1991.tb01662.x","title":"Group V allergens in grass pollens. II. Investigation of group V allergens in pollens from 10 grasses","abstract":"<jats:title>Summary</jats:title><jats:p>In an earlier study an allergen from <jats:italic>Phleum pratense</jats:italic> (timothy) pollen. <jats:italic>Phl p</jats:italic> V. has been isolated and physicochemically characterized. In this study <jats:italic>Phl p</jats:italic> V and immunochemically simitar components from other grass pollens (group V allergens) have been investigated using immunoelectrophoretic techniques. To study the allergenic importance of the group V allergens, the allergenic compositions of 10 grass pollen extracts were investigated in crossed radioimmunoelectrophoresis (CRIE) using 20 sera from grass pollen‐allergic donors. Group V allergens were identified using monospecific rabbit antibodies raised against <jats:italic>Phl p</jats:italic> V, anti‐<jats:italic>phl p</jats:italic> V, which react with other group V allergens usually producing dense precipitates in immunoelectrophoresis. In this way group V allergens were identified in eight extracts, and when present the precipitate corresponding to the group V allergen was the dominant IgE binding precipitate. All identified group V allergens bound IgE in at least 17 of the 20 investigated sera. Monospecific rabbit antibodies raised against the group I allergen of <jats:italic>Lolium perenne</jats:italic> (rye grass), <jats:italic>anit‐Lol p</jats:italic> I. do not precipitate group V allergens, indicating that there are no immunochemical similarities between group I and group V allergens. In SDS‐PAGE anti‐<jats:italic>Phl p</jats:italic> V identifies IgE‐binding components with molecular weights between 26 and 33 kD. In contrast, <jats:italic>anti‐Lol p</jats:italic> I binds to components of slightly higher molecular weight. Apparently, the group V components are allergens that are physicochemically and immunochemically distinct from group I allergens.</jats:p>","journal":"Clinical &amp; Experimental Allergy","year":1991,"id":23069,"datarank":6.0515677958328045,"base_score":4.418840607796598,"endowment":4.418840607796598,"self_citation_contribution":0.6628260911694899,"citation_network_contribution":5.388741704663315,"self_endowment_contribution":0.6628260911694899,"citer_contribution":5.388741704663315,"corpus_percentile":null,"corpus_rank":null,"citation_count":82,"citer_count":76,"citers_with_citation_signal":72,"citers_with_endowment":72,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":142929,"name":"H. LøWENSTEIN","orcid":null,"position":1,"is_corresponding":false},{"id":142928,"name":"F. MATTHIESEN","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.418840607796598,"endowment":4.418840607796598,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1863893","pmcid":null,"openalex_id":"https://openalex.org/W1989795226","authors":[],"funders":[],"total_grants":0,"fwci":1.7546,"citation_percentile":0.8518381,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":2}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2222.1991.tb01662.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2222.1991.tb01662.x","host_type":"publisher"},{"url":"https://doi.org/10.1111/j.1365-2222.1991.tb01662.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1863893","host_type":"repository"}],"fields_of_study":["Allergic Rhinitis and Sensitization","Contact Dermatitis and Allergies","Food Allergy and Anaphylaxis Research","Biology","Medicine","Environmental Science","Allergens","Autoradiography","Electrophoresis, Polyacrylamide Gel","Immunoblotting","Immunoelectrophoresis, Two-Dimensional","Immunoglobulin E","Plant Extracts","Poaceae","Pollen"],"mesh_terms":["Allergens","Autoradiography","Electrophoresis, Polyacrylamide Gel","Poaceae","Immunoglobulin E","Immunoelectrophoresis, Two-Dimensional","Plant Extracts","Pollen","Immunoblotting"],"keywords":["Phleum","Allergen","Lolium perenne","Immunoglobulin E","Pollen","Immunoelectrophoresis","Group A","Chemistry","Allergy","Antibody","Immunology","Biology","Botany","Poaceae","Medicine","Internal medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T17:52:48.409255Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}