{"doi":"10.1111/febs.70060","title":"A metastasis‐associated pannexin‐1 mutant (Panx1 <sup>1‐89</sup> ) forms a minimalist <scp>ATP</scp> release channel","abstract":"A truncated form of the ATP release channel pannexin 1 (Panx1), Panx1 1–89 , is enriched in metastatic breast cancer cells and has been proposed to mediate metastatic cell survival by increasing ATP release through mechanosensitive Panx1 channels. However, whether Panx1 1‐89 on its own [without the presence of wild‐type Panx1 (wtPanx1)] mediates ATP release has not been tested. Here, we show that Panx1 1‐89 by itself can form a constitutively active membrane channel, capable of releasing ATP even in the absence of wtPanx1. Our biophysical characterization reveals that most basic structure–function features of the channel pore are conserved in the truncated Panx1 1‐89 polypeptide. Thus, augmenting extracellular potassium ion concentrations enhances Panx1 1‐89 ‐mediated conductance. Moreover, despite the severe truncation, Panx1 1‐89 retains sensitivity to most wtPanx1 channel inhibitors. Therefore, Panx1 blockers may be of therapeutic value to combat metastatic cell survival. Our study both provides a mechanism for ATP release from cancer cells and suggests that Panx1 1‐89 might aid in the structure–function analysis of Panx1 channels.","journal":"FEBS Journal","year":2025,"id":528046,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1384878,"name":"Noah J. Levi","orcid":"0000-0002-2854-9113","position":1,"is_corresponding":false},{"id":1405452,"name":"Maykelis Dı́az","orcid":"0000-0001-8149-2948","position":2,"is_corresponding":false},{"id":680724,"name":"Carsten Mim","orcid":"0000-0001-6402-8270","position":3,"is_corresponding":false},{"id":680726,"name":"Gerhard Dahl","orcid":"0000-0002-1782-8133","position":4,"is_corresponding":false},{"id":999482,"name":"René Barro-Soria","orcid":"0000-0003-4804-2739","position":5,"is_corresponding":false},{"id":649826,"name":"Junjie Wang","orcid":"0000-0002-6169-8145","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T02:50:44.062153Z","pmid":"40087867","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}