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A combinatorial approach involving the introduction of compensatory mutations and manipulation of the stability threshold by chaperone buffering during directed evolution can improve the functional adaptation of a protein, thereby fostering our ability to attain ever‐more ambitious protein functions in the laboratory.</jats:p>","journal":"The FEBS Journal","year":2013,"id":46256,"datarank":3.9711410369903777,"base_score":4.61512051684126,"endowment":4.61512051684126,"self_citation_contribution":0.692268077526189,"citation_network_contribution":3.2788729594641888,"self_endowment_contribution":0.692268077526189,"citer_contribution":3.2788729594641888,"corpus_percentile":null,"corpus_rank":null,"citation_count":100,"citer_count":95,"citers_with_citation_signal":85,"citers_with_endowment":85,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":214793,"name":"Nobuhiko Tokuriki","orcid":null,"position":1,"is_corresponding":false},{"id":214792,"name":"Raymond D. 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