{"doi":"10.1111/exd.13774","title":"Gelatin as a convenient surrogate protein to model the in vitro effects of advanced glycation end‐product formation","abstract":"<jats:title>Abstract</jats:title><jats:p>Protein glycation has been implicated in skin ageing and several other disease states; however, the slow rate of glycation end‐product formation makes in vitro studies challenging and often impractical. Gelatin, a denatured form of collagen, was identified as a convenient glycation surrogate amenable to cell culture conditions. The suitability of glycated gelatin to model the effects of AGE formation was verified using RAW 264.7 macrophages which revealed a remarkable correlation to previously documented effects. Effects of glycated gelatin on the central role of NF‐ĸB and its downstream consequences (COX‐2 and CD86) confirmed the pro‐inflammatory nature of advanced glycation end‐products. Together, these findings provide confidence that this model could prove a valuable tool to study the poorly understood mechanisms characterizing cellular dysfunction in response to AGE accumulation.</jats:p>","journal":"Experimental Dermatology","year":2018,"id":25133,"datarank":0.620832447432399,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.30891621618042353,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.30891621618042353,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":6,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":148683,"name":"Maryna van de Venter","orcid":null,"position":1,"is_corresponding":false},{"id":148684,"name":"Trevor C. Koekemoer","orcid":null,"position":2,"is_corresponding":false},{"id":148682,"name":"Nadine A. Pringle","orcid":"0000-0001-6940-2356","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30136316","pmcid":null,"openalex_id":"https://openalex.org/W2888391892","authors":[],"funders":[{"funder_name":"Deutscher Akademischer Austauschdienst","grant_id":"DAAD150914142513","title":null},{"funder_name":"National Research Foundation","grant_id":"","title":null},{"funder_name":"South African Medical Research Council","grant_id":"","title":null}],"total_grants":3,"fwci":0.6193,"citation_percentile":0.67300036,"influential_citations":1,"citation_trend":[{"year":2020,"count":2},{"year":2021,"count":2},{"year":2024,"count":3}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fexd.13774","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/exd.13774","host_type":"publisher"},{"url":"https://doi.org/10.1111/exd.13774","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30136316","host_type":"repository"}],"fields_of_study":["Advanced Glycation End Products research","Natural Antidiabetic Agents Studies","Protein Hydrolysis and Bioactive Peptides","Biology","Medicine","Animals","B7-2 Antigen","Cyclooxygenase 2","Gelatin","Glycation End Products, Advanced","Glycosylation","Macrophages","Mice","Models, Biological","Phagocytosis","Protein Transport","RAW 264.7 Cells","Signal Transduction","Transcription Factor RelA"],"mesh_terms":["RAW 264.7 Cells","Animals","Gelatin","Glycosylation","Macrophages","Models, Biological","Phagocytosis","Signal Transduction","Glycation End Products, Advanced","Protein Transport","Mice","Cyclooxygenase 2","B7-2 Antigen","Transcription Factor RelA"],"keywords":["Advanced glycation end-product","Glycation","Gelatin","In vitro","Chemistry","Biochemistry","Receptor","Macrophages","Diabetes","NF-κB","Advanced Glycation End-products"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T00:09:18.647522Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}