{"doi":"10.1111/cup.14308","title":"Pilot study of fluorescence imitating brightfield imaging for rapid, slide‐free dermatopathology","abstract":"BACKGROUND: Fluorescence imitating brightfield imaging (FIBI) is a novel alternative microscopy method that can image freshly excised, non-sectioned tissue. We examine its potential utility in dermatopathology by examining readily available specimens embedded in paraffin blocks. METHODS: Nine skin samples embedded in paraffin blocks were superficially deparaffinized using xylene and ethanol and stained with H&E. FIBI captured tissue surface histopathology images using simple microscope optics and a color camera. We then applied deep-learning-based models to improve resemblance to standard H&E coloration and contrast. FIBI images were compared with corresponding standard H&E slides and concordance was assessed by two dermatopathologists who numerically scored epidermal and dermal structure appearance and overall diagnostic utility. RESULTS: Dermatopathologist scores indicate that FIBI images are at least equivalent to standard H&E slides for visualizing structures such as epidermal layers, sweat glands, and nerves. CONCLUSION: Images acquired with FIBI are comparable to traditional H&E-stained slides, suggesting that this rapid, inexpensive, and non-destructive microscopy technique is a conceivable alternative to standard histopathology processes especially for time-sensitive procedures and in settings with limited histopathology resources.","journal":"Journal of Cutaneous Pathology","year":2022,"id":292076,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.937,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":975757,"name":"Tanishq Mathew Abraham","orcid":"0000-0002-9590-391X","position":1,"is_corresponding":false},{"id":739003,"name":"Taryn Morningstar","orcid":null,"position":2,"is_corresponding":false},{"id":975758,"name":"Maxwell A. Fung","orcid":"0000-0003-1771-8257","position":3,"is_corresponding":false},{"id":976245,"name":"Arshia Rangchi","orcid":null,"position":4,"is_corresponding":false},{"id":147017,"name":"Maija Kiuru","orcid":"0000-0002-7010-5516","position":5,"is_corresponding":false},{"id":908082,"name":"Farzad Fereidouni","orcid":"0000-0001-6790-0888","position":6,"is_corresponding":false},{"id":676598,"name":"Richard M. Levenson","orcid":"0000-0002-0112-6823","position":7,"is_corresponding":false},{"id":975756,"name":"Tess Engel","orcid":"0000-0003-2316-5476","position":0,"is_corresponding":true}],"reference_count":17,"raw_metadata":null,"created_at":"2026-07-19T00:30:42.221508Z","pmid":"36053830","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}