{"doi":"10.1111/ctr.14984","title":"Comparison of two donor‐derived cell‐free DNA tests and a blood gene‐expression profile test in heart transplantation","abstract":"BACKGROUND: Donor-derived cell-free DNA (dd-cfDNA) testing is an emerging screening modality for noninvasive detection of acute rejection (AR). This study compared the testing accuracy for AR of two commercially available dd-cfDNA and gene-expression profiling (GEP) testing in heart transplant (HTx) recipients. METHODS: This is a retrospective, observational study of HTx only patients who underwent standard and expanded single nucleotide polymorphism (SNP) dd-cfDNA between October 2020 to January 2022. Comparison with GEP was also performed. Assays were compared for correlation, accurate classification, and prediction for AR. RESULTS: A total of 428 samples from 112 unique HTx patients were used for the study. A positive standard SNP correlated with the expanded SNP assay (p < .001). Both standard and expanded SNP tests showed low sensitivity (39%, p = 1.0) but high specificity (82% and 84%, p = 1.0) for AR. GEP did not improve sensitivity and showed worse specificity (p < .001) compared to standard dd-cfDNA. CONCLUSION: We found no significant difference between standard and expanded SNP assays in detecting AR. We show improved specificity without change in sensitivity using dd-cfDNA in place of GEP testing. Prospective controlled studies to address how to best implement dd-cfDNA testing into clinical practice are needed.","journal":"Clinical Transplantation","year":2023,"id":336125,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9539,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1053028,"name":"Bryn Gerding","orcid":"0000-0001-7635-0443","position":1,"is_corresponding":false},{"id":1053026,"name":"Vincenzo Cusi","orcid":"0000-0001-9681-6896","position":2,"is_corresponding":false},{"id":423680,"name":"Florin Vaida","orcid":"0000-0002-2256-4611","position":3,"is_corresponding":false},{"id":271800,"name":"Yuko Tada","orcid":"0000-0003-1410-9894","position":4,"is_corresponding":false},{"id":368450,"name":"Gerald P. Morris","orcid":"0000-0002-1097-4453","position":5,"is_corresponding":false},{"id":367214,"name":"Eric Adler","orcid":"0000-0002-4765-0188","position":6,"is_corresponding":false},{"id":240845,"name":"Josef Stehlik","orcid":"0000-0002-7362-0513","position":7,"is_corresponding":false},{"id":854965,"name":"Paul Kim","orcid":"0000-0002-1755-6182","position":8,"is_corresponding":false},{"id":1053027,"name":"Nicholas Rodgers","orcid":"0000-0001-7940-1322","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T01:10:17.243221Z","pmid":"37036133","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}