{"doi":"10.1111/cns.70232","title":"<scp>H4K12</scp>\n                    Lactylation Activated‐\n                    <i>Spp1</i>\n                    in Reprogrammed Microglia Improves Functional Recovery After Spinal Cord Injury","abstract":"<jats:title>ABSTRACT</jats:title>\n                  <jats:sec>\n                    <jats:title>Background</jats:title>\n                    <jats:p>Spinal cord injury (SCI) is a severe condition leading to significant disability and high mortality. The role of the secreted phosphoprotein 1 (SPP1) signaling pathway in SCI, which is quickly activated after injury, is critical for intercellular communication but remains poorly understood.</jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims</jats:title>\n                    <jats:p>This study aimed to explore the function and regulatory mechanisms of the SPP1 signaling pathway in SCI and investigate its potential as a therapeutic target for improving functional recovery after injury.</jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods</jats:title>\n                    <jats:p>\n                      Single‐cell RNA sequencing (scRNA‐seq) was employed to identify ligands and receptors of the SPP1 signaling pathway, particularly in microglia/macrophages. Recombinant SPP1 (rSPP1) was used in vitro and in vivo to assess its effects on neuronal maturation, mitochondrial energy in axons, and functional recovery after SCI. Pseudotime analysis was conducted to examine the role of\n                      <jats:italic>Spp1</jats:italic>\n                      in microglial activation and proliferation. DNA‐pulldown and in vitro experiments were performed to investigate the upstream regulatory proteins of\n                      <jats:italic>Spp1</jats:italic>\n                      .\n                    </jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Results</jats:title>\n                    <jats:p>\n                      The SPP1 signaling pathway is primarily localized in microglia after SCI, with rSPP1 promoting neuronal maturation and enhancing mitochondrial function in axons. Injection of rSPP1 into the injured spinal cord resulted in significant improvement in functional recovery. Pseudotime analysis indicated that\n                      <jats:italic>Spp1</jats:italic>\n                      is involved in the activation and proliferation of microglia. Histone H4 lysine 12 lactylation (H4K12la) was found to promote the transcription of\n                      <jats:italic>Spp1</jats:italic>\n                      in reprogrammed microglia postinjury.\n                    </jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion</jats:title>\n                    <jats:p>\n                      Our findings reveal a novel regulatory mechanism involving\n                      <jats:italic>Spp1</jats:italic>\n                      in SCI, particularly its role in microglial activation, mitochondrial function, and glycolytic reprogramming. This new insight provides a deeper understanding of its contribution to the injury response.\n                    </jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion</jats:title>\n                    <jats:p>\n                      This study uncovers a previously unreported mechanism of\n                      <jats:italic>Spp1</jats:italic>\n                      in SCI, offering a potential therapeutic target for SCI.\n                    </jats:p>\n                  </jats:sec>","journal":"CNS Neuroscience &amp; Therapeutics","year":2025,"id":651598,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1699447,"name":"Geliang Zhou","orcid":null,"position":1,"is_corresponding":false},{"id":1699448,"name":"Junjun Xiong","orcid":null,"position":2,"is_corresponding":false},{"id":1699449,"name":"Wu Ye","orcid":null,"position":3,"is_corresponding":false},{"id":470080,"name":"Yu Gao","orcid":"0000-0001-5090-8250","position":4,"is_corresponding":false},{"id":1699450,"name":"Haofan Wang","orcid":null,"position":5,"is_corresponding":false},{"id":1699451,"name":"Dishui Pan","orcid":null,"position":6,"is_corresponding":false},{"id":1699452,"name":"Yongjun Luo","orcid":null,"position":7,"is_corresponding":false},{"id":486471,"name":"Zheng Zhou","orcid":"0009-0001-7176-1566","position":8,"is_corresponding":false},{"id":237549,"name":"Xiaokun Wang","orcid":"0000-0003-4148-5561","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"<scp>H4K12</scp>\n                    Lactylation Activated‐\n                    <i>Spp1</i>\n                    in Reprogrammed Microglia Improves Functional Recovery After Spinal Cord Injury","abstract":"<jats:title>ABSTRACT</jats:title>\n                  <jats:sec>\n                    <jats:title>Background</jats:title>\n                    <jats:p>Spinal cord injury (SCI) is a severe condition leading to significant disability and high mortality. The role of the secreted phosphoprotein 1 (SPP1) signaling pathway in SCI, which is quickly activated after injury, is critical for intercellular communication but remains poorly understood.</jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims</jats:title>\n                    <jats:p>This study aimed to explore the function and regulatory mechanisms of the SPP1 signaling pathway in SCI and investigate its potential as a therapeutic target for improving functional recovery after injury.</jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods</jats:title>\n                    <jats:p>\n                      Single‐cell RNA sequencing (scRNA‐seq) was employed to identify ligands and receptors of the SPP1 signaling pathway, particularly in microglia/macrophages. Recombinant SPP1 (rSPP1) was used in vitro and in vivo to assess its effects on neuronal maturation, mitochondrial energy in axons, and functional recovery after SCI. Pseudotime analysis was conducted to examine the role of\n                      <jats:italic>Spp1</jats:italic>\n                      in microglial activation and proliferation. DNA‐pulldown and in vitro experiments were performed to investigate the upstream regulatory proteins of\n                      <jats:italic>Spp1</jats:italic>\n                      .\n                    </jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Results</jats:title>\n                    <jats:p>\n                      The SPP1 signaling pathway is primarily localized in microglia after SCI, with rSPP1 promoting neuronal maturation and enhancing mitochondrial function in axons. Injection of rSPP1 into the injured spinal cord resulted in significant improvement in functional recovery. Pseudotime analysis indicated that\n                      <jats:italic>Spp1</jats:italic>\n                      is involved in the activation and proliferation of microglia. Histone H4 lysine 12 lactylation (H4K12la) was found to promote the transcription of\n                      <jats:italic>Spp1</jats:italic>\n                      in reprogrammed microglia postinjury.\n                    </jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion</jats:title>\n                    <jats:p>\n                      Our findings reveal a novel regulatory mechanism involving\n                      <jats:italic>Spp1</jats:italic>\n                      in SCI, particularly its role in microglial activation, mitochondrial function, and glycolytic reprogramming. This new insight provides a deeper understanding of its contribution to the injury response.\n                    </jats:p>\n                  </jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion</jats:title>\n                    <jats:p>\n                      This study uncovers a previously unreported mechanism of\n                      <jats:italic>Spp1</jats:italic>\n                      in SCI, offering a potential therapeutic target for SCI.\n                    </jats:p>\n                  </jats:sec>","is_dataset_classified":null,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39939834","pmcid":"PMC11821456","openalex_id":"https://openalex.org/W4407403003","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"82302794","title":null},{"funder_name":"China Postdoctoral Science Foundation","grant_id":"2023M741469","title":null},{"funder_name":"Natural Science Foundation of Jiangsu Basic Research Program-Youth Fund Project","grant_id":"BK20220734","title":null},{"funder_name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","grant_id":"KYCX23_1949","title":null},{"funder_name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","grant_id":"SJCX24_0789","title":null}],"total_grants":5,"fwci":10.2377,"citation_percentile":0.98734671,"influential_citations":0,"citation_trend":[{"year":2025,"count":6},{"year":2026,"count":7}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1111/cns.70232","host_type":"journal"},{"url":"https://doi.org/10.1111/cns.70232","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/cns.70232","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1111/cns.70232","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39939834","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11821456","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11821456","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11821456?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Spinal Cord Injury Research","Neuroinflammation and Neurodegeneration Mechanisms","Neurogenesis and neuroplasticity mechanisms"],"mesh_terms":["Animals","Cells, Cultured","Female","Histones","Male","Mice, Inbred C57BL","Spinal Cord Injuries","Signal Transduction","Rats, Sprague-Dawley","Microglia","Recovery of Function","Mice","Osteopontin","Cellular Reprogramming"],"keywords":["Microglia","Spinal cord injury","Cell biology","Biology","Signal transduction","Neuroscience","Medicine","Spinal cord","Immunology","Inflammation","Metabolic Reprogramming","Spp1","Histone Lactylation"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T10:06:12.779580Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}