{"doi":"10.1111/all.16651","title":"Mast Cells Are Intrinsically Primed for Degranulation in <scp>DOCK8</scp> Deficiency","abstract":"The data that support the findings of this study are available from the corresponding author upon reasonable request. Table S1. Characteristics of DOCK8-deficient patients. Table S2. Proteins that were observed in fetal skin-derived cultured mast cells (FSCMCs) from DOCK8 mutant mice (Dock8pri/pri mice) and littermate controls. Proteins included if ≥ 3 unique peptides and if the protein was found in ≥ 1 sample. Figure S1. Mcpt5-Cre+; Dock8fl/fl mice exhibit a slight reduction in mast cell numbers in the ear pinna. (A) Quantification of Dock8 mRNA in fetal skin-derived cultured mast cells (FSCMCs) generated from littermate controls and Mcpt5-Cre+; Dock8fl/fl mice. (B–C) Number of mast cells in the ear pinna (B) and dorsal skin (C) of controls (n = 9) and Mcpt5-Cre+; Dock8fl/fl mice (n = 14–16) as quantified in paraffin sections stained with 0.1% Toluidine blue. (D) Percentage of peritoneal mast cells (FcεRIα+; c-Kit+) in the live cell population isolated from controls (n = 4) and Mcpt5-Cre+; Dock8fl/fl mice (n = 6) as analyzed by flow cytometry. (E–I) Percentages of basophils (FcεRIα+; CD49b+; c-Kit−) (E), neutrophils (Ly6G+; F4/80−) (F), spleen T cells (CD3+, CD4+, CD8+) (G), B cells (B220+) (H), and macrophages (F4/80+, Ly6G−) (I) in the live cell population isolated from controls (n = 4) and Mcpt5-Cre+; Dock8fl/fl mice (n = 5–6) as analyzed by flow cytometry. Data in A-I are shown as means + SEM with squares representing values for individual mice. *p < 0.05; **p < 0.01. Figure S2. Representative confocal images of the actin cytoskeleton of naïve peripheral blood mononuclear cell-derived cultured mast cells (PBCMCs) obtained from two DOCK8-deficient patients (Patients 4 and 5) and an age-matched control. Confocal images show staining for actin (red) and nuclei (blue). Scale bar equals 10 μm. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. 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