{"doi":"10.1111/all.15363","title":"Similar <scp>IgE</scp> binding patterns in Gulf of Mexico and Southeast Asian shrimp species in <scp>US</scp> shrimp allergic patients","abstract":"Shellfish allergy (SA) is a leading cause of food-induced anaphylaxis1 and one of the most common causes of adult-onset food allergy worldwide, with 1%–3% of the United States (US) population affected.2-4 Nearly half (45%) of US adults with SA report utilizing emergency services for SA symptoms over their lifetime,2 remaining at-risk for lethal allergic reactions. Several allergenic proteins have been identified across shellfish species, including tropomyosin (TM), arginine kinase (AK), myosin light chain (MLC), sarcoplasmic calcium-binding protein (SCP), hemocyanin, troponin C, and triosephosphate isomerase.5 (Table 1.) However, there are a large number of shrimp allergens that have been detected, but not yet characterized.6 The allergens of major importance in SA are the muscle proteins TM and AK. TM, the major allergen with specific-IgE antibodies in ≤90% of SA patients, is associated with severe clinical reactivity. AK is a pan-allergen with cross-reactivity with crustaceans and cephalopods.5 Cha f 1, Cra c 1, Hom a 1, Lit v 1, Mel l 1, Met e 1, Pan b 1, Pen m 1, Por p 1 Ingestion Inhalation Litopenaeus vannamei (White leg shrimp) Lit v Ingestion Inhalation Penaeus aztecus (Brown shrimp) Pen a Homarus americanus (American lobster) Hom a Crangon crangon (Sand shrimp) Cra c Charybdis feriatus (Crucifix crab) Cha f Ingestion Inhalation Portunus pelagicus (Blueswimmer crab) Por p Cross-reactivity has been observed clinically when SA patients ingest various invertebrate species with subsequent allergic reactions, but further study of shrimp sIgE binding between different shrimp species is needed.7, 8 This study examined the sIgE binding patterns to 2 shrimp species from the Gulf of Mexico and Southeast Asia in US SA patients. Shellfish allergy patients with a history of shrimp-induced allergic reactions, allergic reaction with clinical oral food challenge and positive immediate skin prick testing (IHST) and/or shrimp sIgE ImmunoCAP™ levels were recruited from the Baylor College of Medicine (BCM) Allergy and Immunology Clinics. The study was approved by the BCM IRB and all participants provided written, informed consent. The patients underwent IHST to shrimp extract (mixture of Penaeus borealis, Penaeus monodon, Metapenaeus barbata, and Metapenaeopsis joyner), Dermatophagoides pteronyssinus (Der p1, Der p 10), Dermatophagoides farinae (Der f1), cockroach, codfish, crab, lobster, and oyster using extracts from Greer™. The patients underwent prick and prick IHST to raw fresh shrimp (Penaeus aztecus), and cooked fresh shrimp (Penaeus aztecus). ImmunoCAP™ and ISAC™ customized testing by ThermoFisher™ assessed total IgE as well as sIgE levels for shrimp, recombinant Penaeus aztecus (TM), Der p10, Der p1, Der p2, recombinant Penaeus monodon AK, MLC, SCP, troponin C, crab, lobster, cockroach, clam, and oyster. Western blot (WB) analysis of sIgE binding profile to the major allergenic proteins TM, AK, MLC, and SCP in Penaeus aztecus (Brown shrimp) and Penaeus monodon (Black tiger shrimp) in raw and boiled extracts was performed. Fifteen SA patients between the ages of 2–17 years were enrolled. (Table 2) On oral food challenge, the most frequent symptoms were urticaria and angioedema. Forty percent had antihistamine treatment, one required steroids, and none required epinephrine or albuterol. When examining sIgE binding patterns to the major allergens, WB revealed nearly identical sIgE binding profiles to tropomyosin, MLC, and SCP in both species in the heated shrimp extracts. (Figure 1) Eight subjects (53%) demonstrated sIgE binding to TM and six to SCP (40%). There was variability in the binding to other shrimp proteins, particularly in the unheated shrimp.5 The proposed sIgE binding allergens have been identified by molecular weight,5, 6 and mass spectrometric in-gel analysis, (Table S1.) but need to be confirmed in future molecular studies.9, 10 The presence of Southeast Asian Black tiger shrimp sIgE has been established and quantifi","journal":"Allergy","year":2022,"id":284900,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.968,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":963560,"name":"Shea Brunner","orcid":null,"position":1,"is_corresponding":false},{"id":963561,"name":"Karen S. Tuano","orcid":null,"position":2,"is_corresponding":false},{"id":824957,"name":"Bin Su","orcid":"0000-0002-7236-9857","position":3,"is_corresponding":false},{"id":963089,"name":"Shaymaviswanathan Karnaneedi","orcid":"0000-0003-2384-2625","position":4,"is_corresponding":false},{"id":275203,"name":"Andreas L. Lopata","orcid":"0000-0002-2940-9235","position":5,"is_corresponding":false},{"id":273994,"name":"Carla M. Davis","orcid":"0000-0003-0866-7822","position":6,"is_corresponding":false},{"id":527403,"name":"Sara Anvari","orcid":"0000-0002-9942-6188","position":0,"is_corresponding":true}],"reference_count":10,"raw_metadata":null,"created_at":"2026-07-19T00:29:40.826853Z","pmid":"35524542","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}