{"doi":"10.1111/aji.13235","title":"Pregnancy associates with alterations to the host and microbial proteome in vaginal mucosa","abstract":"PROBLEM: Pregnant women are at increased risk of HIV acquisition, but the biological mechanisms contributing to this observation are not well understood. METHOD OF STUDY: Here, we assessed host immune and microbiome differences in the vaginal mucosa of healthy pregnant and non-pregnant women using a metaproteomics approach. Cervicovaginal lavage (CVL) samples were collected from 23 pregnant and 25 non-pregnant women. RESULTS: Mass spectrometry analysis of CVL identified 550 human proteins and 376 bacterial proteins from 11 genera. Host proteome analysis indicated 56 human proteins (10%) were differentially abundant (P < .05) between pregnant and non-pregnant women, including proteins involved in angiogenesis (P = 3.36E-3), cell movement of phagocytes (P = 1.34E-6), and permeability of blood vessels (P = 1.27E-4). The major bacterial genera identified were Lactobacillus, Gardnerella, Prevotella, Megasphaera, and Atopobium. Pregnant women had higher levels of Lactobacillus species (P = .017) compared with non-pregnant women. Functional pathway analysis indicated that pregnancy associated with changes to bacterial metabolic pathway involved in energy metabolism, which were increased in pregnant women (P = .035). CONCLUSION: Overall, pregnant women showed differences in the cervicovaginal proteome and microbiome that may be important for HIV infection risk.","journal":"American Journal of Reproductive Immunology","year":2020,"id":71971,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8981,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":240566,"name":"Nicole H. Tobin","orcid":"0000-0001-6904-6128","position":1,"is_corresponding":false},{"id":381837,"name":"Trisha Vera","orcid":null,"position":2,"is_corresponding":false},{"id":381838,"name":"Lani Kotyrba","orcid":null,"position":3,"is_corresponding":false},{"id":315893,"name":"Laura Noël‐Romas","orcid":null,"position":4,"is_corresponding":false},{"id":315890,"name":"Kenzie Birse","orcid":null,"position":5,"is_corresponding":false},{"id":315892,"name":"Sarah Mutch","orcid":null,"position":6,"is_corresponding":false},{"id":314052,"name":"Fan Li","orcid":"0000-0002-2400-9021","position":7,"is_corresponding":false},{"id":260276,"name":"David Lee","orcid":"0000-0003-3346-0970","position":8,"is_corresponding":false},{"id":381839,"name":"Stuart McCorrister","orcid":null,"position":9,"is_corresponding":false},{"id":381840,"name":"Garrett Westmacott","orcid":null,"position":10,"is_corresponding":false},{"id":240568,"name":"Grace M. Aldrovandi","orcid":"0000-0003-1604-9637","position":11,"is_corresponding":false},{"id":314054,"name":"Adam Burgener","orcid":"0000-0002-2303-5738","position":12,"is_corresponding":false},{"id":314043,"name":"Christina Farr Zuend","orcid":"0009-0001-4165-6909","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-18T21:44:45.622639Z","pmid":"32196803","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}