{"doi":"10.1111/acer.14471","title":"Proteomic Profiling Reveals Roles of Stress Response, Ca<sup>2+</sup> Transient Dysregulation, and Novel Signaling Pathways in Alcohol‐Induced Cardiotoxicity","abstract":"BACKGROUND: Alcohol use in pregnancy increases the risk of abnormal cardiac development, and excessive alcohol consumption in adults can induce cardiomyopathy, contractile dysfunction, and arrhythmias. Understanding molecular mechanisms underlying alcohol-induced cardiac toxicity could provide guidance in the development of therapeutic strategies. METHODS: We have performed proteomic and bioinformatic analysis to examine protein alterations globally and quantitatively in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) treated with ethanol (EtOH). Proteins in both cell lysates and extracellular culture media were systematically quantitated. RESULTS: handling. Treatment of hiPSC-CMs with EtOH significantly affected proteins responsible for stress response (e.g., GPX1 and HSPs), ion channel-related proteins (e.g. ATP1A2), myofibril structure proteins (e.g., MYL2/3), and those involved in focal adhesion and extracellular matrix (e.g., ILK and PXN). Proteins involved in the TNF receptor-associated factor 2 signaling (e.g., CPNE1 and TNIK) were also affected by EtOH treatment. CONCLUSIONS: handling and contraction while also implicating potential novel targets in alcohol-induced cardiotoxicity. These findings facilitate further exploration of potential mechanisms, discovery of novel biomarkers, and development of targeted therapeutics against EtOH-induced cardiotoxicity.","journal":"Alcoholism Clinical and Experimental Research","year":2020,"id":89396,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9551,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":339882,"name":"Fangxu Sun","orcid":"0000-0002-4318-479X","position":1,"is_corresponding":false},{"id":451865,"name":"Parvin Forghani","orcid":"0000-0001-9727-3344","position":2,"is_corresponding":false},{"id":452428,"name":"Lawrence C. Armand","orcid":null,"position":3,"is_corresponding":false},{"id":339883,"name":"Antonio Rampoldi","orcid":"0000-0003-2494-5925","position":4,"is_corresponding":false},{"id":451866,"name":"Li Dong","orcid":"0009-0005-1574-068X","position":5,"is_corresponding":false},{"id":339885,"name":"Ronghu Wu","orcid":"0000-0001-9493-9462","position":6,"is_corresponding":false},{"id":339887,"name":"Chunhui Xu","orcid":"0000-0003-1748-0648","position":7,"is_corresponding":false},{"id":339880,"name":"Rui Liu","orcid":"0000-0003-2661-7183","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-18T22:01:50.225871Z","pmid":"32981093","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}