{"doi":"10.1111/1758-2229.13234","title":"Inactivation of siderophore iron‐chelating moieties by the fungal wheat root symbiont\n                    <i>Pyrenophora biseptata</i>","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    We investigated the ability of four plant and soil‐associated fungi to modify or degrade siderophore structures leading to reduced siderophore iron‐affinity in iron‐limited and iron‐replete cultures.\n                    <jats:italic>Pyrenophora biseptata</jats:italic>\n                    , a melanized fungus from wheat roots, was effective in inactivating siderophore iron‐chelating moieties. In the supernatant solution, the tris‐hydroxamate siderophore desferrioxamine B (DFOB) underwent a stepwise reduction of the three hydroxamate groups in DFOB to amides leading to a progressive loss in iron affinity. A mechanism is suggested based on the formation of transient ferrous iron followed by reduction of the siderophore hydroxamate groups during fungal high‐affinity reductive iron uptake.\n                    <jats:italic>P. biseptata</jats:italic>\n                    also produced its own tris‐hydroxamate siderophores (neocoprogen I and II, coprogen and dimerum acid) in iron‐limited media and we observed loss of hydroxamate chelating groups during incubation in a manner analogous to DFOB. A redox‐based reaction was also involved with the tris‐catecholate siderophore protochelin in which oxidation of the catechol groups to quinones was observed. The new siderophore inactivating activity of the wheat symbiont\n                    <jats:italic>P. biseptata</jats:italic>\n                    is potentially widespread among fungi with implications for the availability of iron to plants and the surrounding microbiome in siderophore‐rich environments.\n                  </jats:p>","journal":"Environmental Microbiology Reports","year":2024,"id":620232,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1601057,"name":"Emmanuel Chukwuma","orcid":null,"position":1,"is_corresponding":false},{"id":1601060,"name":"Ilan Linshitz","orcid":null,"position":2,"is_corresponding":false},{"id":1601063,"name":"Kosuke Namba","orcid":null,"position":3,"is_corresponding":false},{"id":692705,"name":"Owen W. Duckworth","orcid":"0000-0002-1453-7402","position":4,"is_corresponding":false},{"id":1141303,"name":"Marc A. Cubeta","orcid":"0000-0001-9595-5894","position":5,"is_corresponding":false},{"id":879813,"name":"Oliver Baars","orcid":"0000-0002-4644-5086","position":6,"is_corresponding":false},{"id":1601055,"name":"Katie S. French","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Inactivation of siderophore iron‐chelating moieties by the fungal wheat root symbiont\n                    <i>Pyrenophora biseptata</i>","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    We investigated the ability of four plant and soil‐associated fungi to modify or degrade siderophore structures leading to reduced siderophore iron‐affinity in iron‐limited and iron‐replete cultures.\n                    <jats:italic>Pyrenophora biseptata</jats:italic>\n                    , a melanized fungus from wheat roots, was effective in inactivating siderophore iron‐chelating moieties. In the supernatant solution, the tris‐hydroxamate siderophore desferrioxamine B (DFOB) underwent a stepwise reduction of the three hydroxamate groups in DFOB to amides leading to a progressive loss in iron affinity. A mechanism is suggested based on the formation of transient ferrous iron followed by reduction of the siderophore hydroxamate groups during fungal high‐affinity reductive iron uptake.\n                    <jats:italic>P. biseptata</jats:italic>\n                    also produced its own tris‐hydroxamate siderophores (neocoprogen I and II, coprogen and dimerum acid) in iron‐limited media and we observed loss of hydroxamate chelating groups during incubation in a manner analogous to DFOB. A redox‐based reaction was also involved with the tris‐catecholate siderophore protochelin in which oxidation of the catechol groups to quinones was observed. The new siderophore inactivating activity of the wheat symbiont\n                    <jats:italic>P. biseptata</jats:italic>\n                    is potentially widespread among fungi with implications for the availability of iron to plants and the surrounding microbiome in siderophore‐rich environments.\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38240404","pmcid":"PMC10866069","openalex_id":null,"authors":[],"funders":[{"funder_name":"Novo Nordisk Fonden","grant_id":"#NNF19SA0059360","title":null},{"funder_name":"National Science Foundation","grant_id":"1757699","title":null},{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF19SA0059360","title":null},{"funder_name":"Hatch Project","grant_id":"NC02713","title":null},{"funder_name":"United States Department of Agriculture-National Institute of Food and Agriculture","grant_id":"2019-06522","title":null},{"funder_name":"State of North Carolina","grant_id":"","title":null}],"total_grants":6,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/1758-2229.13234","host_type":"publisher"},{"url":"https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.13234","host_type":"publisher"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10866069/pdf/EMI4-16-e13234.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10866069","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10866069?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Ascomycota","Triticum","Iron","Siderophores","Iron Chelating Agents"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T10:27:12.494546Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}