{"doi":"10.1111/1348-0421.12922","title":"<i>cydA</i>, <i>spdC</i>, and <i>mroQ</i> are novel genes involved in the plasma coagulation of <i>Staphylococcus aureus</i>","abstract":"<jats:title>Abstract</jats:title><jats:p>Coagulation is a critical pathogenic process in <jats:italic>Staphylococcus aureus</jats:italic>. Although the agglutination of <jats:italic>S. aureus</jats:italic> has been studied for a long time, the genes involved in this process are not completely clear. We performed tube agglutination and dynamic turbidimetry tests to identify novel genes involved in reduced plasma coagulation. A total of 15 genes were identified, including <jats:italic>coa</jats:italic>, <jats:italic>clfA</jats:italic>, <jats:italic>vwbp</jats:italic>, <jats:italic>saeS</jats:italic>, <jats:italic>agrA</jats:italic>, <jats:italic>trpC</jats:italic>, <jats:italic>spdC</jats:italic>, <jats:italic>mroQ</jats:italic>, <jats:italic>cydA</jats:italic>, <jats:italic>qoxC</jats:italic>, <jats:italic>sucC</jats:italic>, <jats:italic>pyrP</jats:italic>, <jats:italic>menH</jats:italic>, <jats:italic>threonine aldolase</jats:italic>, and <jats:italic>truncated transposase for IS1272</jats:italic>. The functions of these genes include bicomponent regulation, membrane transport, energy metabolism and biosynthesis, respectively. <jats:italic>cydA</jats:italic>, <jats:italic>spdC</jats:italic>, and <jats:italic>mroQ</jats:italic> genes were further studied by gene knockout and complementation. Results of gene knockout and complementation and real‐time‐qPCR proved that <jats:italic>cydA</jats:italic>, <jats:italic>spdC</jats:italic>, and <jats:italic>mroQ</jats:italic> genes are necessary for plasma coagulation. Furthermore, the survival ability of 7 day mice decreased significantly when <jats:italic>cydA</jats:italic>, <jats:italic>spdC</jats:italic>, and <jats:italic>mroQ</jats:italic> genes had been knocked out.</jats:p>","journal":"Microbiology and Immunology","year":2021,"id":650471,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":542963,"name":"Wei Wang","orcid":"0000-0001-8676-1190","position":1,"is_corresponding":false},{"id":1194125,"name":"Qiang Chen","orcid":"0000-0003-1410-1278","position":2,"is_corresponding":false},{"id":1696064,"name":"Linfeng Peng","orcid":"0000-0002-7404-6509","position":3,"is_corresponding":false},{"id":722605,"name":"Xiaomei Hu","orcid":"0000-0003-2831-1241","position":4,"is_corresponding":false},{"id":1696065,"name":"Kaisen Chen","orcid":"0000-0003-1498-2538","position":5,"is_corresponding":false},{"id":912197,"name":"Dong Luo","orcid":"0000-0002-9711-2070","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"<i>cydA</i>, <i>spdC</i>, and <i>mroQ</i> are novel genes involved in the plasma coagulation of <i>Staphylococcus aureus</i>","abstract":"<jats:title>Abstract</jats:title><jats:p>Coagulation is a critical pathogenic process in <jats:italic>Staphylococcus aureus</jats:italic>. Although the agglutination of <jats:italic>S. aureus</jats:italic> has been studied for a long time, the genes involved in this process are not completely clear. We performed tube agglutination and dynamic turbidimetry tests to identify novel genes involved in reduced plasma coagulation. A total of 15 genes were identified, including <jats:italic>coa</jats:italic>, <jats:italic>clfA</jats:italic>, <jats:italic>vwbp</jats:italic>, <jats:italic>saeS</jats:italic>, <jats:italic>agrA</jats:italic>, <jats:italic>trpC</jats:italic>, <jats:italic>spdC</jats:italic>, <jats:italic>mroQ</jats:italic>, <jats:italic>cydA</jats:italic>, <jats:italic>qoxC</jats:italic>, <jats:italic>sucC</jats:italic>, <jats:italic>pyrP</jats:italic>, <jats:italic>menH</jats:italic>, <jats:italic>threonine aldolase</jats:italic>, and <jats:italic>truncated transposase for IS1272</jats:italic>. The functions of these genes include bicomponent regulation, membrane transport, energy metabolism and biosynthesis, respectively. <jats:italic>cydA</jats:italic>, <jats:italic>spdC</jats:italic>, and <jats:italic>mroQ</jats:italic> genes were further studied by gene knockout and complementation. Results of gene knockout and complementation and real‐time‐qPCR proved that <jats:italic>cydA</jats:italic>, <jats:italic>spdC</jats:italic>, and <jats:italic>mroQ</jats:italic> genes are necessary for plasma coagulation. Furthermore, the survival ability of 7 day mice decreased significantly when <jats:italic>cydA</jats:italic>, <jats:italic>spdC</jats:italic>, and <jats:italic>mroQ</jats:italic> genes had been knocked out.</jats:p>","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34050992","pmcid":null,"openalex_id":"https://openalex.org/W3165280084","authors":[],"funders":[],"total_grants":0,"fwci":0.3098,"citation_percentile":0.5820522,"influential_citations":0,"citation_trend":[{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1111/1348-0421.12922","host_type":"journal"},{"url":"https://doi.org/10.1111/1348-0421.12922","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/1348-0421.12922","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1348-0421.12922","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34050992","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9292280","host_type":"repository"}],"fields_of_study":["Antimicrobial Resistance in Staphylococcus","Bacterial biofilms and quorum sensing","Bacterial Genetics and Biotechnology"],"mesh_terms":["Agglutination","Animals","Bacterial Proteins","Coagulase","Staphylococcal Infections","Staphylococcus aureus","Mice"],"keywords":["Biology","Complementation","Gene","Staphylococcus aureus","Genetics","Microbiology","Bacteria"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Affordable and clean energy"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T05:32:19.242373Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}