{"doi":"10.1110/ps.073328208","title":"Rational design of a novel calcium‐binding site adjacent to the ligand‐binding site on CD2 increases its CD48 affinity","abstract":"<jats:title>Abstract</jats:title><jats:p>Electrostatic interactions are important for molecular recognition processes including Ca<jats:sup>2+</jats:sup>‐binding and cell adhesion. To understand these processes, we have successfully introduced a novel Ca<jats:sup>2+</jats:sup>‐binding site into the non‐Ca<jats:sup>2+</jats:sup>‐dependent cell adhesion protein CD2 using our criteria that are specifically tailored to the structural and functional properties of the protein environment and charged adhesion surface. This designed site with ligand residues exclusively from the β‐sheets selectively binds to Ca<jats:sup>2+</jats:sup> and Ln<jats:sup>3+</jats:sup> over other mono‐ and divalent cations. While Ca<jats:sup>2+</jats:sup> and Ln<jats:sup>3+</jats:sup> binding specifically alters the local environment of the designed Ca<jats:sup>2+</jats:sup>‐binding site, the designed protein undergoes a significantly smaller conformation change compared with those observed in naturally occurring Ca<jats:sup>2+</jats:sup>‐binding sites that are composed of at least part of the flexible loop and helical regions. In addition, the CD2–CD48‐binding affinity increased approximately threefold after protein engineering, suggesting that the cell adhesion of CD2 can be modulated by altering the local electrostatic environment. The study provides site‐specific information for regulating cell adhesion within CD2 and gives insight into the structural factors required for Ca<jats:sup>2+</jats:sup>‐modulated biological processes.</jats:p>","journal":"Protein Science","year":2008,"id":615881,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":362179,"name":"Wei Yang","orcid":"0000-0003-3785-1450","position":1,"is_corresponding":false},{"id":1587617,"name":"Anna W. 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To understand these processes, we have successfully introduced a novel Ca<jats:sup>2+</jats:sup>‐binding site into the non‐Ca<jats:sup>2+</jats:sup>‐dependent cell adhesion protein CD2 using our criteria that are specifically tailored to the structural and functional properties of the protein environment and charged adhesion surface. This designed site with ligand residues exclusively from the β‐sheets selectively binds to Ca<jats:sup>2+</jats:sup> and Ln<jats:sup>3+</jats:sup> over other mono‐ and divalent cations. While Ca<jats:sup>2+</jats:sup> and Ln<jats:sup>3+</jats:sup> binding specifically alters the local environment of the designed Ca<jats:sup>2+</jats:sup>‐binding site, the designed protein undergoes a significantly smaller conformation change compared with those observed in naturally occurring Ca<jats:sup>2+</jats:sup>‐binding sites that are composed of at least part of the flexible loop and helical regions. In addition, the CD2–CD48‐binding affinity increased approximately threefold after protein engineering, suggesting that the cell adhesion of CD2 can be modulated by altering the local electrostatic environment. The study provides site‐specific information for regulating cell adhesion within CD2 and gives insight into the structural factors required for Ca<jats:sup>2+</jats:sup>‐modulated biological processes.</jats:p>","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18287277","pmcid":"PMC2248323","openalex_id":"https://openalex.org/W2018235755","authors":[],"funders":[{"funder_name":"Medical Research Council","grant_id":"G19/31","title":null},{"funder_name":"Medical Research Council","grant_id":"G9722488","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM 62999-1","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R21 GM070555","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM070555","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM062999","title":null}],"total_grants":6,"fwci":1.3009,"citation_percentile":0.78474356,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":2},{"year":2015,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1110/ps.073328208","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1110/ps.073328208","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1110%2Fps.073328208","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1110/ps.073328208","host_type":"publisher"},{"url":"https://doi.org/10.1110/ps.073328208","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18287277","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2248323","host_type":"repository"},{"url":"https://ora.ox.ac.uk/objects/uuid:fcb3c639-ccc7-40ce-baa1-0c9687e397d1","host_type":"repository"}],"fields_of_study":["Lipid Membrane Structure and Behavior","Cell Adhesion Molecules Research","Monoclonal and Polyclonal Antibodies Research","Antigens, CD","Binding Sites","CD2 Antigens","CD48 Antigen","Calcium","Cations","Cell Adhesion","Lanthanum","Ligands","Models, Molecular","Mutagenesis, Site-Directed","Static Electricity","Temperature"],"mesh_terms":["CD48 Antigen","Binding Sites","Calcium","Cations","Cell Adhesion","Lanthanum","Ligands","Models, Molecular","Temperature","Antigens, CD","Mutagenesis, Site-Directed","CD2 Antigens","Static Electricity"],"keywords":["Binding site","Divalent","Biophysics","Chemistry","Ligand (biochemistry)","Adhesion","Rational design","Cell adhesion","Plasma protein binding","A-site","Calcium-binding protein","Protein structure","Calcium","Biochemistry","Cell","Nanotechnology","Biology","Materials science","Receptor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T21:20:16.291544Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}