{"doi":"10.1109/tmi.2025.3643618","title":"Multifocal Optical-Resolution Photoacoustic Microscopy With a Masked Single-Element Transducer","abstract":"Optical-resolution photoacoustic microscopy (OR-PAM) can visualize cellular-level wavelength-dependent optical absorption with high resolution and sensitivity. However, the imaging speed of OR-PAM has been limited by the laser repetition rate due to the point-by-point scanning of a focused laser beam. To overcome this limitation, we propose multifocal optical-resolution photoacoustic microscopy (MOR-PAM) with a single-element ultrasonic transducer, leveraging the diffractive optical element (DOE) and a custom-designed encoding acoustic mask. The DOE generates 8 focal spots of $3~\\mu $ m diameter. The acoustic mask was designed to encode photoacoustic signals from different focal spots. MOR-PAM achieved an 8-fold increase in imaging speed compared to conventional OR-PAM with the same laser repetition rate. We demonstrated the MOR-PAM using a 266 nm laser at 10 KHz, providing solutions for rapid OR-PAM beyond the laser repetition rate in a cost-effective way. The proposed method can be applied to versatile OR-PAM configurations and enable new applications where high-speed imaging is critical.","journal":"IEEE Transactions on Medical Imaging","year":2025,"id":585179,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9564,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":473756,"name":"Rui Cao","orcid":"0000-0003-4444-7528","position":1,"is_corresponding":false},{"id":345459,"name":"Peng Hu","orcid":"0000-0002-2933-1239","position":2,"is_corresponding":false},{"id":668181,"name":"Yilin Luo","orcid":"0000-0002-4611-3049","position":3,"is_corresponding":false},{"id":845639,"name":"Yushun Zeng","orcid":"0000-0001-5995-4085","position":4,"is_corresponding":false},{"id":585749,"name":"Yide Zhang","orcid":"0000-0002-9463-3970","position":5,"is_corresponding":false},{"id":1176278,"name":"Manxiu Cui","orcid":"0000-0003-0788-9511","position":6,"is_corresponding":false},{"id":266837,"name":"Qifa Zhou","orcid":"0000-0003-1527-3020","position":7,"is_corresponding":false},{"id":476225,"name":"Geng Ku","orcid":null,"position":8,"is_corresponding":false},{"id":253575,"name":"Lihong V. Wang","orcid":"0000-0001-9783-4383","position":9,"is_corresponding":false},{"id":1176276,"name":"Xiaofei Luo","orcid":"0000-0002-3005-4240","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:59:20.067334Z","pmid":"41385414","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}