{"doi":"10.1109/tbme.2023.3237267","title":"Comprehensive Surface Histology of Fresh Resection Margins With Rapid Open-Top Light-Sheet (OTLS) Microscopy","abstract":"Objective: For tumor resections, margin status typically correlates with patient survival but positive margin rates are generally high (up to 45% for head and neck cancer). Frozen section analysis (FSA) is often used to intraoperatively assess the margins of excised tissue, but suffers from severe under-sampling of the actual margin surface, inferior image quality, slow turnaround, and tissue destructiveness. Methods: Here, we have developed an imaging workflow to generate en face histologic images of freshly excised surgical margin surfaces based on open-top light-sheet (OTLS) microscopy. Key innovations include (1) the ability to generate false-colored H&E-mimicking images of tissue surfaces stained for < 1 min with a single fluorophore, (2) rapid OTLS surface imaging at a rate of 15 min/cm 2 followed by real-time post-processing of datasets within RAM at a rate of 5 min/cm 2 , and (3) rapid digital surface extraction to account for topological irregularities at the tissue surface. Results: In addition to the performance metrics listed above, we show that the image quality generated by our rapid surface-histology method approaches that of gold-standard archival histology. Conclusion: OTLS microscopy has the feasibility to provide intraoperative guidance of surgical oncology procedures. Significance: The reported methods can potentially improve tumor-resection procedures, thereby improving patient outcomes and quality of life.","journal":"IEEE Transactions on Biomedical Engineering","year":2023,"id":335853,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9576,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1065843,"name":"Dominie Miyasato","orcid":"0000-0001-8977-7411","position":1,"is_corresponding":false},{"id":560155,"name":"Lindsey A. Barner","orcid":"0000-0002-9156-0024","position":2,"is_corresponding":false},{"id":323421,"name":"Robert Serafin","orcid":"0000-0002-6646-353X","position":3,"is_corresponding":false},{"id":353034,"name":"Kevin W. Bishop","orcid":"0000-0001-8722-6464","position":4,"is_corresponding":false},{"id":323422,"name":"Weisi Xie","orcid":"0000-0002-2576-9583","position":5,"is_corresponding":false},{"id":279202,"name":"Adam K. Glaser","orcid":"0000-0003-3558-8994","position":6,"is_corresponding":false},{"id":270918,"name":"Eben L. Rosenthal","orcid":"0000-0003-0294-5203","position":7,"is_corresponding":false},{"id":35293,"name":"Lawrence D. True","orcid":"0000-0002-8621-9569","position":8,"is_corresponding":false},{"id":20776,"name":"Jonathan Liu","orcid":"0000-0001-5650-3086","position":9,"is_corresponding":false},{"id":560158,"name":"Gan Gao","orcid":"0000-0002-8100-7735","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T01:10:02.777338Z","pmid":"37021859","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}