{"doi":"10.1109/access.2020.2994943","title":"Automated in Vivo Assessment of Vascular Response to Radiation Using a Hybrid Theranostic X-Ray Irradiator/Fluorescence Molecular Imaging System","abstract":"Hypofractionated stereotactic body radiotherapy treatments (SBRT) have demonstrated impressive results for the treatment of a variety of solid tumors. The role of tumor supporting vasculature damage in treatment outcome for SBRT has been intensely debated and studied. Fast, non-invasive, longitudinal assessments of tumor vasculature would allow for thorough investigations of vascular changes correlated with SBRT treatment response. In this paper, we present a novel theranostic system which incorporates a fluorescence molecular imager into a commercial, preclinical, microCT-guided, irradiator and was designed to quantify tumor vascular response (TVR) to targeted radiotherapy. This system overcomes the limitations of single-timepoint imaging modalities by longitudinally assessing spatiotemporal differences in intravenously-injected ICG kinetics in tumors before and after high-dose radiation. Changes in ICG kinetics were rapidly quantified by principle component (PC) analysis before and two days after 10 Gy targeted tumor irradiation. A classifier algorithm based on PC data clustering identified pixels with TVR. Results show that two days after treatment, a significant delay in ICG clearance as measured by exponential decay (40.5±16.1% P=0.0405 Paired t-test n=4) was observed. Changes in the mean normalized first and second PC feature pixel values (PC1 & PC2) were found (P=0.0559, 0.0432 paired t-test), suggesting PC based analysis accurately detects changes in ICG kinetics. The PC based classification algorithm yielded spatially-resolved TVR maps. Our first-of-its-kind theranostic system, allowing automated assessment of TVR to SBRT, will be used to better understand the role of tumor perfusion in metastasis and local control.","journal":"IEEE Access","year":2020,"id":107995,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9624,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":455012,"name":"Jamison Brooks","orcid":"0009-0001-9468-9878","position":1,"is_corresponding":false},{"id":396655,"name":"Darren Zuro","orcid":null,"position":2,"is_corresponding":false},{"id":455015,"name":"Srideshikan Sargur Madabushi","orcid":"0000-0002-1087-6044","position":3,"is_corresponding":false},{"id":423042,"name":"Dayson Moreira","orcid":null,"position":4,"is_corresponding":false},{"id":421919,"name":"Marcin Kortylewski","orcid":"0000-0002-6003-1816","position":5,"is_corresponding":false},{"id":455018,"name":"Jerry Froelich","orcid":"0000-0002-1972-9868","position":6,"is_corresponding":false},{"id":309281,"name":"Min‐Ying Su","orcid":"0000-0002-3069-0271","position":7,"is_corresponding":false},{"id":425391,"name":"Gültekin Gülşen","orcid":null,"position":8,"is_corresponding":false},{"id":342355,"name":"Susanta Hui","orcid":"0000-0002-1394-3724","position":9,"is_corresponding":false},{"id":424427,"name":"Farouk Nouizi","orcid":"0000-0001-5395-2505","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T23:12:39.385256Z","pmid":"32542176","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}