{"doi":"10.1107/s2053230x23000705","title":"Purification and structure of luminal domain C of human Niemann–Pick C1 protein","abstract":"Niemann–Pick C1 protein (NPC1) is a membrane protein that primarily resides in late endosomes and lysosomes, and plays an important role in cholesterol homeostasis in the cell. The second luminal domain of NPC1 (NPC1-C) serves as the intracellular receptor for Ebola and Marburg viruses. Here, the recombinant production of nonglycosylated and glycosylated NPC1-C and a new crystal form of the nonglycosylated protein are reported. The crystals belonged to space group P 2 1 and diffracted to 2.3 Å resolution. The structure is similar to other reported structures of NPC1-C, with differences observed in the protruding loops when compared with NPC1-C in complex with Ebola virus glycoprotein or NPC2.","journal":"Acta Crystallographica Section F Structural Biology Communications","year":2023,"id":374197,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9473,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":562724,"name":"Kaneil K. Zadrozny","orcid":null,"position":1,"is_corresponding":false},{"id":284587,"name":"William E. Diehl","orcid":"0000-0001-7943-1214","position":2,"is_corresponding":false},{"id":284590,"name":"Jeremy Luban","orcid":"0000-0001-5650-4054","position":3,"is_corresponding":false},{"id":315224,"name":"Judith M. White","orcid":"0000-0002-0532-996X","position":4,"is_corresponding":false},{"id":255582,"name":"Barbie K. Ganser‐Pornillos","orcid":"0000-0001-6453-3891","position":5,"is_corresponding":false},{"id":315225,"name":"Lukas K. Tamm","orcid":"0000-0002-1674-4464","position":6,"is_corresponding":false},{"id":255584,"name":"Owen Pornillos","orcid":"0000-0001-9056-5002","position":7,"is_corresponding":false},{"id":653511,"name":"Laura Odongo","orcid":"0000-0002-9096-7965","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T01:16:11.341687Z","pmid":"36748341","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}