{"doi":"10.1107/s2052252520012701","title":"Harnessing the power of an X-ray laser for serial crystallography of membrane proteins crystallized in lipidic cubic phase","abstract":"Serial femtosecond crystallography (SFX) with X-ray free-electron lasers (XFELs) has proven highly successful for structure determination of challenging membrane proteins crystallized in lipidic cubic phase; however, like most techniques, it has limitations. Here we attempt to address some of these limitations related to the use of a vacuum chamber and the need for attenuation of the XFEL beam, in order to further improve the efficiency of this method. Using an optimized SFX experimental setup in a helium atmosphere, the room-temperature structure of the adenosine A 2A receptor (A 2A AR) at 2.0 Å resolution is determined and compared with previous A 2A AR structures determined in vacuum and/or at cryogenic temperatures. Specifically, the capability of utilizing high XFEL beam transmissions is demonstrated, in conjunction with a high dynamic range detector, to collect high-resolution SFX data while reducing crystalline material consumption and shortening the collection time required for a complete dataset. The experimental setup presented herein can be applied to future SFX applications for protein nanocrystal samples to aid in structure-based discovery efforts of therapeutic targets that are difficult to crystallize.","journal":"IUCrJ","year":2020,"id":79003,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9611,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":411020,"name":"James H. Geiger","orcid":"0000-0002-9443-4488","position":1,"is_corresponding":false},{"id":411021,"name":"Andrii Ishchenko","orcid":"0000-0002-3797-0604","position":2,"is_corresponding":false},{"id":266169,"name":"Gye Won Han","orcid":"0000-0003-4107-835X","position":3,"is_corresponding":false},{"id":412441,"name":"Anton Barty","orcid":null,"position":4,"is_corresponding":false},{"id":411022,"name":"Thomas A. White","orcid":"0000-0002-8407-0270","position":5,"is_corresponding":false},{"id":198891,"name":"Cornelius Gati","orcid":"0000-0003-3693-7009","position":6,"is_corresponding":false},{"id":240877,"name":"A. Batyuk","orcid":"0000-0002-9393-2880","position":7,"is_corresponding":false},{"id":282896,"name":"Mark S. Hunter","orcid":"0000-0002-0110-7075","position":8,"is_corresponding":false},{"id":282893,"name":"Andrew Aquila","orcid":"0000-0003-0358-2774","position":9,"is_corresponding":false},{"id":282903,"name":"Sébastien Boutet","orcid":"0000-0003-3928-1244","position":10,"is_corresponding":false},{"id":411023,"name":"Uwe Weierstall","orcid":"0000-0002-3594-930X","position":11,"is_corresponding":false},{"id":198890,"name":"Vadim Cherezov","orcid":"0000-0002-5265-3914","position":12,"is_corresponding":false},{"id":411024,"name":"Wei Liu","orcid":"0000-0002-1727-6738","position":13,"is_corresponding":false},{"id":292042,"name":"Ming-Yue Lee","orcid":"0000-0001-7082-1216","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:50:33.748601Z","pmid":"33209312","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}