{"doi":"10.1107/s1744309108040682","title":"Crystallization and preliminary crystallographic analysis of the second RRM of Pub1 from<i>Saccharomyces cerevisiae</i>","abstract":"mRNA stability is elaborately regulated by elements in the mRNA transcripts and their cognate RNA-binding proteins, which play important roles in regulating gene expression at the post-transcriptional level in eukaryotes. Poly(U)-binding protein 1 (Pub1), which is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae, is involved in the regulation of mRNA turnover as a trans-acting factor. It binds to transcripts containing the AU-rich element in order to protect them from degradation. Pub1 contains three RNA-recognition motifs (RRMs) which play significant roles in mRNA binding at AU-rich elements and stabilizer elements. In this study, the second RRM of Pub1 was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 4000 as a precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group H3.","journal":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","year":2009,"id":8879,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0423,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2009-01-07","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":30887,"name":"Alexandra P. Lewis","orcid":"0000-0002-6195-4786","position":1,"is_corresponding":false},{"id":59043,"name":"Xu Li","orcid":"0000-0002-4640-2529","position":2,"is_corresponding":false},{"id":28483,"name":"Yang Li","orcid":"0000-0003-4022-7341","position":3,"is_corresponding":false},{"id":76022,"name":"Huihao Zhou","orcid":"0000-0002-9675-5007","position":4,"is_corresponding":false},{"id":76023,"name":"Liwen Niu","orcid":"0000-0003-1217-1782","position":5,"is_corresponding":false},{"id":76024,"name":"Maikun Teng","orcid":"0000-0003-4621-826X","position":6,"is_corresponding":false},{"id":76021,"name":"Yingji Cui","orcid":null,"position":0,"is_corresponding":true}],"reference_count":11,"raw_metadata":null,"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}