{"doi":"10.1105/tpc.108.200310","title":"The IMEter Predicts an Intron's Ability to Boost Gene Expression","abstract":null,"journal":"The Plant Cell","year":2008,"id":635132,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1647649,"name":"Kathleen L. Farquharson","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The IMEter Predicts an Intron's Ability to Boost Gene Expression","abstract":"Most eukaryotic genes are interrupted by one or more introns that are transcribed and then removed by splicing before the mRNA exits the nucleus. Some intron-containing genes have much higher expression levels than intronless versions of the same gene (reviewed in Le Hir et al., 2003). The first intron of the shrunken-1 gene from maize produced a 91-fold increase in expression of a reporter gene construct (Vasil et al., 1989). Enhancing introns must fall within 1 kb of the transcription start site to have a positive effect on mRNA accumulation (Rose, 2004). However, the mechanism underlying intron-mediated enhancement (IME) in plants is largely unknown. Sequence motifs that may be responsible for IME in Arabidopsis and rice. To determine if IME signals are conserved across plant species, the researchers trained the IMEter on more than 30,000 introns from rice and again tested its ability to detect expression-enhancing introns in Arabidopsis. Once more, the calculated IMEter scores were in good agreement with the observed expression-enhancing abilities of the introns. This suggests that the signals responsible for IME are conserved between Arabidopsis and rice. The rice-trained IMEter also predicted enhancing introns from maize and rice, and further analysis identified an IME signal in rice that is similar to the enhancing motif of Arabidiopsis (see Figure). Because addition of an intron from the host species can often enhance transgene expression, the authors suggest that the IMEter may be a useful tool for optimizing expression in transgenic plants.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":"https://openalex.org/W2163285239","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.13093239,"influential_citations":0,"citation_trend":[{"year":2016,"count":1}],"oa_status":"green","license":"http://aspb.org/publications/aspb-journals/open-articles","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2329940","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2329940","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1105/tpc.108.200310","host_type":"publisher"},{"url":"https://doi.org/10.1105/tpc.108.200310","host_type":"journal"}],"fields_of_study":["RNA Research and Splicing","RNA and protein synthesis mechanisms","RNA modifications and cancer"],"mesh_terms":[],"keywords":["Intron","Biology","RNA splicing","Gene","Genetics","Gene expression","Precursor mRNA","Mature messenger RNA","Messenger RNA","Nucleus","Cell biology","RNA"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:41:04.505007Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}